Antioxidative effects of fluvastatin and its metabolites in cultured human endothelial cells using single cell gel electrophoresis

概要

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In the present study, we investigated the antioxidative effects of fluvastatin, a 3-hydroxy-3-methylglutaryl coenzyme A reductase inhibitor, and its metabolites (M2, M3, and M4) on oxidative DNA damage in cultured human umbilical vein endothelial cells (HUVEC), as well as the effects of other inhibitors of this enzyme, pravastatin and simvastatin. Single cell gel electrophoresis was used to evaluate the protective effects of fluvastatin on reactive oxygen species (ROS)-induced DNA damage in HUVEC exposed to either t-butylhydroperoxide or hydrogen peroxide. Fluvastatin and its metabolites showed protective effects on DNA damage as potent as the reference antioxidants, ascorbic acid, trolox, and probucol. It was suggested that fluvastatin has the potential to protect cells against ROS-induced DNA damage, although pravastatin and simvastatin did not exhibit clear protective effects. Fluvastatin may contribute to reduce ROS in humans and also reduce ROS related diseases.
日本環境変異原学会の論文
2002-05-31