KK-42 処理除脳蛹を使った前胸腺刺激ホルモン生物検定法

概要

論文の詳細を見る
Improvement of tbe bioassay method for prothoracicotropic hormone (PTTH) in the silkworm, Bombyx mori, was achieved. When an imidazole compound (KK-42 : 1-benzyl-5-[(E)-2,6-dimethy-1 , 5-heptadienyl] imidazole) was applied on the pupae with brains extirpated just after pupation, the pupal-adult development was completely inhibited in almost all of the animals investigated. Although dauer pupae (artificially diapausing pupae) could be prepared only by the extirpation of brains just after pupation in a specific race of Bombyx (Japanese No. 122 × Chinese No. 115), these animals initiated adult development spontaneously 30 to 50 days after the operation. In the case of the improved method, pupal-adult development was arrested more than 90 days after the treatments. The advantages of the improved method for PTTH assay are as follows : 1) arrested states of the animals are steady, 2) many races of Bombyx can be used for PTTH assay, 3) it is easy to prepare the dauer pupae in Bombyx, and 4) sensitivity of the animals for the exogeneous PTTH is higher than that of the debrained animals used so far. It is conclusively demonstrated that brainless pupae treated with the imidazole compound are useful materials for the bioassay of PTTH.
日本応用動物昆虫学会の論文
1990-05-25