カイコガ成虫頭部糖蛋白質の部分精製とその生理活性

概要

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Glycoproteins were extracted and partially purified from heads of adults of the silkworm, Bombyx mori. Physiological activity of the glycoproteins was also examined using five different bioassay systems. The yield of the glycoproteins separated by ConA-Sepharose 4B affinity chromatography was about 43 mg from 120 g of the starting materials. The molecular weights of the main glycoproteins were 15,000,30,000 and 50,000 as determined by gel-filtration on Sephadex G-75. Neuro-hormonal activities (prothoracicotropic hormones, diapause hormcne, eclosion hormone) were not detected in the glycoprotein fraction. Effect of the glycoprotein fraction on the ovarian development of isolated abdomens was investigated, but no gonadotropic action could be detected : the ovaries grew only when 20-hydroxyecdysone was injected into the isolated abdomens.
日本応用動物昆虫学会の論文
1987-08-25