イネの芽ばえを用いたニカメイチュウの簡易大量累代飼育方法

概要

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This paper describes a technique for mass rearing of rice stem borer, Chilo suppressalis for successive generations on rice seedlings in glass bottles (diameter 10 cm, depth 10 cm). The rice seedlings were germinated from 50g of rice seeds in the glass bottle, and fifty hatchlings were placed in a bottle and allowed to feed. The larvae were transferred to a new glass bottle with the seedlings after 15 days from the hatch. The pupae were transferred into a plastic box from the glass bottle, and adults emerged were removed to an oviposition net cage, in which the potted rice plant was provided as oviposition site. This experiment was carried out at 25℃ with a day length of 15 hours. The results of rearing are summarized in the table. Chemical constitutions of the mature larvae reared are as follows : water content, 67.6 per cent in live material ; crude fat content, 41.4 per cent and total nitrogen content, 4.77 per cent in dry material. Median lethal dosage of parathion applied topically on mature larvae is 3.30μg per gram of the body weight. These physiological properties were consistent with those obtained from the larvae in the field, indicating that this rearing method is well suited for the practical purpose.
日本応用動物昆虫学会の論文
1964-03-25