Isoelectric focusing electrophoresis and RFLP analysis : Two methods for immature Orius spp. identification

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概要

• 論文の詳細を見る

• Two techniques were used to discriminate between second instar Orius minutus (L) and Orius sauteri (Poppius) which occur sympatrically in eggplant fields of Japan. Isoelectric focusing (IEF) was used to discriminate between the two species utilizing the enzyme isocitrate dehydrogenase (IDH). Moreover, IEF was also shown to detect the presence of the prey Thrips palmi Karny in O. sauteri nymphs immediately after feeding by using phosphogluco-isomerase (PGI). PCR generated internal transcribed spacer I (ITS-1) regions for nymphs of both species were also diagnostic as the O. minutus PCR product was much larger than the O. sauteri PCR product (900 vs. 600 bp). Moreover, by comparing ITS-1 PCR product sizes and using the restriction enzyme HinfI on ITS-1 products of similar size to pr.oduce restriction fragment length polymorphisms (RFLPs), consistent differences among five Japanese Orius species were also demonstrated.

• 日本応用動物昆虫学会の論文
• 1999-02-25

著者

• TAKAGI Masami

  Institute of Biological Control, Faculty of Agriculture, Graduate School, Kyushu University

• Takagi M

  Institute Of Biological Control Faculty Of Agriculture Kyushu University

• Takagi Masami

  Institute Of Biological Control Faculty Of Agriculture Kushu University

• Takagi Masami

  Institute Of Biological Control Faculty Of Agriculture Graduate School Kyushu University

• Takagi M

  Faculty Of Agriculture Kyushu University

• HIROSE Yoshimi

  Institute of Biological Control, Faculty of Agriculture, Kushu University

• Nakashima Yoshitaka

  Institute of Biological Control, Faculty of Agriculture, Kyushu University

• Honda Jeffrey

  Department of Biological Sciences, San Jose State University

• Yanase Tohru

  Institute of Biological Control, Faculty of Agriculture, Kyushu University

• Kawarabata Takeshi

  Institute of Biological Control, Faculty of Agriculture, Kyushu University

• Yanase Tohru

  Institute Of Biological Control Faculty Of Agriculture Kyushu University

• Honda Jeffrey

  Department Of Biological Sciences San Jose State University

• Hirose Y

  Kyushu Univ. Fukuoka Jpn

• Nakashima Y

  Institute Of Biological Control Faculty Of Agriculture Kyushu University

• Nakashima Yoshitaka

  Institute Of Biological Control Faculty Of Agriculture Kyushu University

• Nakashima Yoshitaka

  Laboratory Of Entomology Obihiro University Of Agriculture And Veterinary Medicine

• Kusakabe Takahiro

  Laboratory Of Silkworm Science Faculty Of Agriculture Graduate School Kyushu University

• Kawarabata T

  Laboratory Of Silkworm Science Kyushu University Graduate School Of Bioresource And Bioenvironmental

• Hirose Yoshimi

  Institute Of Biological Control Faculty Of Agriculture Kyushu University

• Kawarabata Takeshi

  Institute Of Biological Control Faculty Of Agriculture Kyushu University

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