Isolation and Structure of a Nuclear Polyhedrosis Virus from Polyhedra of the Silkworm, Bombyx mori

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概要

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• Rate-zonal centrifugation of virus particles released from the alkaline solution of inclusion bodies from the silkworm larva showed that the nuclear polyhedra contained at least three different forms of the virus particles. These forms of the virus were separated in sucrose density gradient centrifugation. The best result of separation was obtained when Trisbuffer was used as gradient buffer. The nuclear polyhedra in the silkworm larva contained 80% of single and 20% of bundle virus rods. All of the separated virus had a developmental membrane. Detail studies on the relative infectivity of both the single and the bundle virus rods showed that they were almost equally infectious in hemocoelic inoculation to the silkworm pupa. The naked virus rod, i.e., virus particle having only an intimate membrane did not occur significantly under the present condition of investigation.

• 日本応用動物昆虫学会の論文
• 1973-12-25

著者

• Matsumoto Keigo

  Institute Of Agricultural Chemicals Sankyo Co.

• Kawarabata Takeshi

  Institute of Biological Control, Faculty of Agriculture, Kyushu University

• Kawarabata Takeshi

  Institute Of Biological Control Faculty Of Agriculture Kyushu University

• 河原畑 勇

  Institute of Biological Control, Faculty of Agriculture, Kyushu University

• 松本 至吾

  Institute of Agricultural Chemicals, Sankyo Co.

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• Susceptibility of Lepidopteran Cell Lines to a Spodoptera exigua (Lepidoptera: Noctuidae) Nuclear Polyhedrosis Virus
• Comparison of Chorion Structure of Eggs from Bombyx mori, Bombyx mandarina (Lepidoptera: Bombycidae) and Their First Filial Generation
• Isolation and Structure of a Nuclear Polyhedrosis Virus from Polyhedra of the Silkworm, Bombyx mori
• Sequencing of the p143 gene encoding a putative DNA helicase of the Spodoptera exigua (Lepidoptera : Noctuidae) nucleopolyhedrovirus I1 isolate from Japan
• Replication of Xestia c-nigrum (Lepidoptera : Noctuidae) Nuclear Polyhedrosis Virus in Continuous Cell Cultures
• Purification of Hafnium by Hydrogen Plasma Arc Melting
• Biology of Microsporidians Infecting the Silkworm, Bombyx mori, in Japan

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