メチシリン耐性黄色ブドウ球菌(MRSA)のメチシリン耐性遺伝子の由来とその発現機構に関する研究

概要

論文の詳細を見る
Analysis of nucleotide sequences of the mecA genes from various MRSA (methicillin-resistant Staphylococcus aureus) showed a unique junction sequence at which the putative ancestral genes, i. e., an inducible penicillinase gene and a penicillin-binding protein gene, are supposed to have been fused to form the mecA gene. Results strongly suggest that the formation of the methicillin-resistant staphylococcus was a unique event and has occurred long before the use of methicillin, considering the world-wide distribution of the mecA gene. Sequencing of the upstream region of mecA showed an open reading frame called mecB located head to head to mecA with a spacing sequence of 100 bp between them. This contained promoters on both strands and consensus palindrome sequences for the repressor-binding. The mecB gene of a MRSA strain TK 784 was sequenced and the protein was analyzed by in vitro protein synthesis followed by SDS-polyacrylamide gel electrophoresis.
東海大学の論文
1993-03-30