<原著>パルス電磁場刺激の骨芽細胞および骨芽細胞前駆細胞への影響 : 無血清培養条件下での細胞外基質タンパクmRNA発現変化

概要

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Pulsing electromagnetic fields (PEMF) are known to be effective in stimulation of nonunion fractures, however the mechanism of osteogenic action of PEMF has not been fully established. This study investigated the effects of PEMF on the gene expression in human osteoblastic and the human mesencymal stem cells (hMSC). The cells were exposed to the PEMF in serum-free medium and the mRNA level of the expression of extraceller matrix associated proteins were examined by quantitative RT-PCR assay using a LightCycler. For the spreading osteoblastic cells on a type I collagen surface with PEMF stimulation, there was an approximately 2 fold increase compared with the expression in the control group for BMP-2 mRNA expression. The addition of 10 ng/ml of TGF beta-1 enhanced the BMP-2 mRNA expression in the hMSC. However, PEMF exposure inhibited BMP-2 mRNA expression in hMSC under this condition. The results indicate that the mechanism of osteogenic action of PEMF may be different in different cell types or in the degree of differentiating potency. Consequently, it is implied that PEMF has the ability to regulate BMP-2 mRNA expression directly in osteogenic cells.
北海道医療大学の論文
2003-06-30