<原著>PDGF-BBとIGF-Iがラット歯髄由来線維芽細胞に及ぼす影響

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Dental pulp cells are able to differentiate into odontoblasts and to form dentin when stimulated by external irritation. Growth factors such as platelet-derived growth factor (PDGF), insulin-like growth factor (IGF), and transforming growth factor-β (TGF-β) as well as bone morphogenetic proteins (BMPs) have been implicated in the regulation of the wound healing of pulp in vitro, but the effects of these growth factors on the function of dental pulp fibroblasts in vitro still remain to be established. This study examined the effects of the best characterized growth factors in mesenchymal cells, PDGF-BB and IGF-I on cell proliferation, cell differentiation, chemotactic activity and the gene expression of other growth factors, such as BMP-1〜5 and TGF-β1 in rat pulpfibroblasts in vitro. The results are as follows. 1. Both PDGF-BB and IGF-I significantly stimulated cell proliferation and chemotactic activity in rat pulp fibroblasts in vitro, and more pronounced effects were observed in PDGF-BB. 2. Mitogenic doses of PDGF-BB inhibited the alkaline phosphatase (ALP) activity, significantly, while mitogenic doses of IGF-I did not have significant effect on ALP. 3. Short term treatment with mitogenic doses of IGF-I increased mRNA levels of BMP-4 and TGF-β1 in,a dose-dependent manner, while long term treatment did not result in significant effects on the mRNA levels of BMPs and TGF-β1. 4. There were no significant effects on the gene expression of BMP-1〜5 and TGF-β1, when the cells were treated with PDGF-BB. These results indicate that both PDGF-BB and IGF-I are mitogenic to rat dental pulpfibroblasts, but that they act in different manners to regulate the function of the dental pulp.
北海道医療大学の論文
1997-12-30

<原著>PDGF-BBとIGF-Iがラット歯髄由来線維芽細胞に及ぼす影響

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