<原著>ESRスピントラッピング法によるスーパーオキシドラジカル測定法の再評価

概要

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-FR80). A quantitative analysis of DMPO-O^-_2 was performed by ESR spectrometry under the conditions of center field: 334.5 mT; sweep width: ±5 mT; modulation width: 0.5×0.1 mT; receiver gain: 4.0×100; and time constant: 0.1 sec. The ESR spin was recorded on a spectrometer (JEOL JES-REIX) using an aqueous quarts flat cell (JEL LC-12, inner size 60 mm×10 mm×0.31mm) with an effective volume of 160 μl. After recording, the signal intensity of the lowest field peak of the spectrum was normalized as the relative height against the standard signal intensity of the manganese oxide (MnO) marker. All results obtained from the present experiment showed that the optimum XOD concentration was 0.3 U/ml, the optimum quantity of DMPO was 15 μl and the optimum time of reaction was within 4 minutes after addition of XOD.
北海道医療大学の論文
1993-12-31