糸状性細菌へ添加された鉄の分布と呼吸阻害効果

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Distribution of iron on/in Sphaerotilus after adding various iron compounds was examined in association with the respiratory inhibition. Prior to detect the sorbed location of iron on/in the microbe, separation of the cellular substances from the sheaeh was carried out by a modified treatment of Romano & Peroquin's method wherein EDTA and lysozyme were added separately in oeder of treatment. The sorption of iron was not observed on/in the microbe in the case of non-inhibitory compounds such as K_3Fe(CN)_6,K_4Fe(CN)_6 and Fe-citrate. But, some bulky layer of hydrous iron oxide occurred covering the sheath surface by adding FeCl_3 which could not inhibit the respiration. For a while, the iron-sorbed layer covering the sheath surface by a dose of Fe-cysteine appeared so much tight to restrict the permeation of outer substrates into the cell. The existance of the added iron was not detected in the cell of the microbe. Such distributional feature of iron might contribute to the characteristic effect of Fe-cysteine which principally inhibited the exogenous repiration. By addition of K_2FeO_4,a fairly dense layer of hydrous iron oxide occurred simnlsly on the sheath, and, futermore the iron permeated through he membrane was surely detected in the cellular substances separated from the sheath by means of the succeeding treatment with lysozyme. The penetrated iron might probably attack to damage the enzymic system relating to respiration of the microbe. Then, these distributional pattern of iron as observed above might connect closely with clarifying the reason that the K_2FeO_4 acted a role of effective inhibitor on the exogenous and endogenous rspiration.
山梨大学の論文

糸状性細菌へ添加された鉄の分布と呼吸阻害効果

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