HeLa細胞のリン脂質代謝に及ぼすcolchicineと12-O-tetradecanoylphorbol-13-acetateの影響

概要

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Colchicine, a microtubule-disrupting agent, and 12-O- tetradecanoylphorbol-13-acetate(TPA), a potent tumor promoter, stimulated the incorporation of choline into phospholipids of HeLa cells. Concurrent addition of colchicine with TPA increased the stimulatory effect of TPA. Vinblastine, a microtubule-interacting agent, showed similar effect to colchicine. However, cytochalasin B, a microfilament-interacting agent, and 1-methyl-l-cyclohexane-carboxylic acid, an intermediate-sized filament-interacting agent, were ineffective. Thus, synergistic effect with TPA was caused specifically by microtubule-interacting agents. It might be possible that microtubule involve in the regulation of the expression of the effect of TPA on the metabolism of phosphatidylcholine. Although the incorporation of inositol into phospholipids was also increased by the treatment with TPA, colchicine did not stimulate inositol incorporation, but inhibited. Furthermore colchicine decreased the stimulatory effect of TPA on inositol incorporation. However, it is unclear whether microtubules are involved in the mechanism of TPA action on phosphatidylinositol metabolism. In any case, these results indicate that the metabolism of each phospholipid is regulated in a different fashion in detail.
園田学園女子大学の論文
1983-10-20