大豆タンパク中亜鉛の有効利用性に対するキレート剤の効果

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Bioavailability of zinc contained in soy protein is lower than that in milk casein. We have observed that apparent absorption of dietary zinc in rats fed a diet including 20% of soy protein isolate as protein and zinc source for one month, was lower than that from milk casein diet. Soy protein isolate is containing about 1% of phytate like many other plant origin feedstuff, and it is generally considered that phytate bind with zinc, calcium, magnesium and/or protein fraction of diet in rat small intestine, and form insoluble chelating compound, so that zinc can not be absobed from the small intestine. Some chelating agents such as ethylenediaminetetraacetic acid (EDTA) or citrate bind with zinc and the complex can keep the metal in a soluble form. To clarify the interaction between zinc and phytate in more detail, we studied whether addition of small amount of citrate or EDTA to the soy protein diet does increare absorption and bioavailability of dietary zinc, or not. Rats were ad libitum fed one of three experimental diets for 31 days. One was a 20% soy protein diet, and the other two were prepared by adding 1% of citric acid or 0.01% of EDTA to the 20% soy protein diet. All diets were containing about 8 ppm of zinc from soy protein isolate. During the experiment, feces excreted from animals were collected and food consumption was measured every day. Zinc in collected feces was analyzed by atomic absorption spectrophotometer, and apparent absorption rate of dietary zinc was calculated. At the end of the feeding period, rats were killed and zinc content in blood, liver, kidney, spleen, femur and tibia was determined.
金城学院大学の論文
1988-03-20

大豆タンパク中亜鉛の有効利用性に対するキレート剤の効果

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