Microfluorometric assay for quantification of anti-erythrocytic antibody level in sera of Plasmodium berghei ANKA infected semi-immune mice

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概要

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• A simple, sensitive and reproducible fluorometry method was developed to quantify anti-erythrocytic auto-antibody in the sera of semi-immune mice. The level of anti-erythrocytic auto-antibody measured using fluorometry was significantly higher in sera of Plasmodium berghei ANKA infected mice than the uninfected, p‹0.0001. This result correlates significantly with the standard ELISA assay, p‹0.0001, r2=0.7766. Both methods show significant correlation with regard to extent of Hb loss, p‹0.006 (r2=0.33) (fluorometry method) and p‹0.0001, r2=0.30 (ELISA). Thus the fluorometry method may serve as an alternative to standard ELISA assay and can be modified to suit similar objectives.

• The Japanese Society of Tropical Medicine,日本熱帯医学会の論文
• 2010-12-01

著者

• Kikuchi Mihoko

  Department Of Immunogenetics Institute Of Tropical Medicine (nekken) Nagasaki University

• Huy Nguyen

  Department Of Applied Biology Kyoto Institute Of Technology:department Of Immunogenetics Institute O

• Yanagi Tetsuo

  Department Of Protozoology Institute Of Tropical Medicine (nekken) Nagasaki University

• Hirayama Kenji

  Department Of Clinical Immunology Medical Institute Of Bioregulation Kyushu University

• Huy Nguyen

  Department Of Immunogenetics Institute Of Tropical Medicine (nekken) Nagasaki University

• Yasunami Michio

  Department Of Immunogenetics Institute Of Tropical Medicine (nekken) Nagasaki University

• HELEGBE Gideon

  Department of Immunogenetics, Institute of Tropical Medicine (NEKKEN), Nagasaki University

• YANAGI Tetsuo

  Animal Research Center for Tropical Infections, Institute of Tropical Medicine (NEKKEN), Nagasaki Un

• SHUAIBU Mohammed

  Department of Immunogenetics, Institute of Tropical Medicine (NEKKEN), Nagasaki University

• Helegbe Gideon

  Department Of Immunogenetics Institute Of Tropical Medicine (nekken) Nagasaki University

• Yanagi Tetsuo

  Animal Research Center For Tropical Infections Institute Of Tropical Medicine (nekken) Nagasaki Univ

• Shuaibu Mohammed

  Department Of Immunogenetics Institute Of Tropical Medicine (nekken) Nagasaki University

• Yanagi Tetsuo

  Animal Research Center For Tropical Infections Institute Of Tropical Medicine (nekken) Nagasaki Univ

• KIKUCHI Mihoko

  Department of Immunogenetics and Global Centre of Excellence, Institute of Tropical Medicine, Nagasaki University

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