Reuber H-35 Cell-Conditioned Medium Prevents Early Degeneration of Bovine Embryos Cultured In Vitro
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概要
- 論文の詳細を見る
The aim of this study was to examine the effects of two kinds of rat hepatoma cell-conditioned media (CM) on in vitro development of bovine embryos matured and fertilized in vitro under chemically defined conditions. Medium conditioned with Reuber H-35 or BRL 3A cells was prepared by a 24-h culture in the absence of both serum and bovine serum albumin (BSA). Two days after in vitro fertilization, bovine embryos were cultured in these conditioned media. After 2 days of culture in the control or in BRL CM, 15-18% of embryos were developed to the morula stage. Although a significant difference was not found, the developmental rate was increased to 22.4% when embryos were cultured in Reuber CM. Additionally, the percentage of degenerated embryos cultured with Reuber CM was significantly decreased (33.5%; p<0.01) compared to that of control embryos (49.4%). These results demonstrated the positive effects of serum- or BSA-free Reuber CM on the inhibition of early degeneration of bovine embryos matured and fertilized in vitro.
- 日本繁殖生物学会の論文
- 1998-11-01
著者
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KOBAYASHI Masayuki
Department of Urology, Graduate School of Medicine, Chiba University
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HORIUCHI RYUYA
Department of Clinical Pharmacology, Gunma University Graduate School of Medicine
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Horiuchi Ryuya
Department Of Clinical Pharmacology And Department Of Pharmacy Gunma University School Of Medicine
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Kobayashi Masayuki
Department Of Animal Science Akita Prefectural College Of Agriculture
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