Plasma Polymerization for Protein Patterning : Reversible Formation with Fullerence Modification
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概要
- 論文の詳細を見る
Partial plasma polymerization for coexistence of hydrophobic/hydrophilic area in several ten micrometer size is the typical technique for protein patterning. A hydrophobic hexamethyldisiloxane plasma-polymerized film (HMDS PPF) was deposited on a glass substrate and this surface was partially modified by subsequent nitrogen plasma treatment (hydrophilic surface, HMDS-N PPF) with a patterned shadow mask. An antibody protein (F(ab)2 fragment of anti-human immunoglobulin G) was selectively adsorbed onto the HMDS-N area and was not adsorbed onto the HMDS area. Distinct 80× 80µm2 square spots surrounded by a non-protein adsorbed 80µm-wide grid were observed. Then, when the protein modified by fullerene was used, the reversible patterning was obtained. This indicated that the modification by fullerene changed the hydrophilic nature of F(ab)2 protein to hydrophobic one, as a result, the modified protein was selectively adsorbed onto hydrophobic area.
- （社）電子情報通信学会の論文
- 2010-02-01
著者
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TAKAHASHI Hayato
Department of Dermatology, Keio University School of Medicine
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Takahashi Hayato
Department Of Electronic Engineering Shibaura Institute Of Technology
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Takahashi Hayato
Department Of Applied Chemistry Faculty Of Science And Technology Keio University
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MURATA Naoya
Department of Electronic Engineering, Shibaura Institute of Technology
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MUGURUMA Hitoshi
Department of Electronic Engineering, Shibaura Institute of Technology
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Murata Naoya
Department Of Electronic Engineering Shibaura Institute Of Technology
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Muguruma Hitoshi
Department Of Electrical And Electronics Engineering Tokyo Institute Of Technology
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