Selective Adsorption of an Antibody onto a Plasma-Polymerized Film for Protein Patterning
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概要
- 論文の詳細を見る
Techniques for patterned modification of substrate surfaces are important for forming microarrays on protein chips. A hexamethyldisiloxane plasma-polymerized film (HMDS PPF) was deposited on a glass substrate and the resulting surface was partially modified by subsequent nitrogen plasma treatment with a patterned shadow mask. When surface adsorption of an antibody protein (F(ab)2 fragment of anti-human immunoglobulin G) was visualized by fluorescence microscopy, distinct 80×80μm2 square spots were observed, surrounded by a non-fluorescent 80μm-wide grid. This pattern could be attributed to proteins selectively adsorbed onto the nitrogen plasma-treated regions but not onto the surface of pristine HMDS PPF. This provided a simple fabrication method of protein patterning.
- （社）電子情報通信学会の論文
- 2008-06-01
著者
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Matsumura Kazunari
Department of Engineering, Shibaura Institute of Technology
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Matsumura Kazunari
Department Of Materials Science And Engineering Shibaura Institute Of Technology
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Matsumura Kazunari
Department Of Chemistry And Biotechnology Graduate School Of Engineering The University Of Tokyo
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HIRATSUKA Atsunori
Research Center of Advanced Bionics, National Institute of Advanced Industrial Science and Technolog
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MURATA Naoya
Department of Electronic Engineering, Shibaura Institute of Technology
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MUGURUMA Hitoshi
Department of Electronic Engineering, Shibaura Institute of Technology
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Murata Naoya
Department Of Electronic Engineering Shibaura Institute Of Technology
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Muguruma Hitoshi
Department Of Electronic Engineering Shibaura Institute Of Technology
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Muguruma Hitoshi
Department Of Electrical And Electronics Engineering Tokyo Institute Of Technology
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Hiratsuka Atsunori
Research Center Of Advanced Bionics National Institute Of Advanced Industrial Science And Technology
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