Isolation, Culture and Biological Characteristics of Primordial Germ Cells from Beijing Fatty Chicken

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概要

• 論文の詳細を見る

• The aim of this study was to explore the isolation and culture process of Beijing fatty chicken primordial germ cells (PGCs) and investigate their biological characteristics. The PGCs isolated from the genital ridges of Beijing fatty chicken (Gallus domesticus) embryos after 5.5 days of incubation were co-cultured with mice embryonic fibroblasts (MEF). The results showed that the PGCs of the Beijing fatty chicken were positive for periodic acid Schiff (PAS) and alkaline phosphatase (AKP) staining. These cells could proliferate for a prolonged time in vitro and maintain diploid karyotype. Immunocytochemical staining showed that they expressed SSEA-1, SSEA-4, TRA-1-60 and TRA-1-81, and real-time PCR showed that they expressed Cvh, CDH and Dazl. They could form simple embryoid bodies and differentiate into osteoblasts in vitro. In addition, after transfected with pEGFP-N3, pEYFP-N1 and pDsRed-N1 vectors by liposomal transfection, enhanced green, yellow and red fluorescent protein-positive cells could be visualized using a laser confocal microscope. The above results suggested that PGCs from the Beijing fatty chicken not only had strong self-renewal ability, but also had the potential to differentiate towards mesoblast cells. These cells are suitable for genetic manipulation as nuclear donors.

• 日本繁殖生物学会の論文
• 2010-06-01

著者

• Ma Yue

  Institute Of Animal Sciences Chinese Academy Of Agricultural Sciences (caas)

• WANG Ying

  Institute of Animal Sciences, Chinese Academy of Agricultural Sciences (CAAS)

• HOU Lingling

  College of Life Sciences and Bioengineering, Beijing Jiaotong University

• LI Chunying

  College of Wildlife Resources, Northeast Forestry University

• GUAN Weijun

  Institute of Animal Sciences, Chinese Academy of Agricultural Sciences (CAAS)

• CHEN Lina

  Institute of Animal Sciences, Chinese Academy of Agricultural Sciences (CAAS)

• LI Xiangchen

  Institute of Animal Sciences, Chinese Academy of Agricultural Sciences (CAAS)

• YUE Wenbin

  College of Animal Science & Technology, Shanxi Agriculture University

• Guan Weijun

  Inst. Of Animal Sciences Chinese Acad. Of Agricultural Sciences (caas)

• Guan Weijun

  Institute Of Animal Sciences Chinese Academy Of Agricultural Sciences (caas)

• Chen Lina

  Institute Of Animal Sciences Chinese Academy Of Agricultural Sciences (caas)

• Hou Lingling

  College Of Life Sciences And Bioengineering Beijing Jiaotong University

• Li Chunying

  College Of Wildlife Resources Northeast Forestry University

• Yue Wenbin

  Coll. Of Animal Sci. & Technol. Shanxi Agriculture Univ.

• Yue Wenbin

  College Of Animal Science & Technology Shanxi Agriculture University

• Li Xiangchen

  Institute Of Animal Sciences Chinese Academy Of Agricultural Sciences (caas)

• Wang Ying

  Institute Of Animal Sciences Chinese Academy Of Agricultural Sciences (caas)

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