Immunohistochemical Demonstration of S-phase Cells in Canine and Feline Spontaneous Tumors by Anti-bromodeoxyuridine Monoclonal Antibody
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概要
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Bromodeoxyuridine (BrdU) labeling of S-phase cells, which are DNA- synthesizing cells, was demonstrated in 30 canine and 6 feline neoplasms. Simultaneously, the optimization of the BrdU-labeling method, including tissue fixation, administration dose of BrdU, and the immunohistochemical procedure, was examined. BrdU-incorporated cells in a neoplasm of a dog administered 3 mg/kg of BrdU could be detected. Although BrdU-positive cells could be visualized in 70% ethanol fixed samples, we could satisfactorily demonstrate BrdU-immunoreactivities in samples fixed in 10% buffered formalin and subjected to enzymatic treatment and acid hydrolysis. The BrdU-labeling index (LI) of malignant neoplasms tended to be higher than that of benign neoplasms. The mean BrdU LI of malignant neoplasms was significantly higher than that of benign neoplasms (P<0.05). The present study demonstrated that the BrdU-labeling method may be useful for detecting S-phase cells in canine and feline tissues.
- 日本毒性病理学会の論文
- 2005-09-30
著者
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高橋 通正
(独)産業技術総合研究所 環境管理技術研究部門
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Tatematsu M
Division Of Oncological Pathology Aichi Cancer Center Research Institute
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SAKAI Hiroki
Laboratory of Oncological Pathology, Aichi Cancer Center Research Institute
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坂井 宏明
帯広畜産大学畜産学部獣医学科家畜内科学教室
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YANAI Tokuma
Laboratory of Veterinary Pathology, Faculty of Applied Biological Sciences, Gifu University
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MASEGI Toshiaki
Laboratory of Veterinary Pathology, Department of Veterinary Medicine, Faculty of Applied Biological
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Yanai T
Department Of Veterinary Pathology Gifu University
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Totsuka Yukari
大阪府立大学先端科学イノベーションセンター 遺伝子環境科学研究室
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Totsuka Y
Cancer Prevention Basic Research Project
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Tatematsu M
愛知県がんセンター研究所 腫瘍病理学部
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高岡 雅哉
Medicinal Safety Research Laboratories Daiichi-sankyo Co. Ltd.
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Tatematsu Masae
愛知県がんセンター研究所 腫瘍病理学部
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Yamachika Takasuke
Division Of Oncological Pathology Aichi Cancer Center Research Institute
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Tajika Masahiro
Department Of Gastroenterology Aichi Cancer Center Hospital
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Takaoka Masaya
Medicinal Safety Research Laboratories Daiichi-sankyo Co. Ltd.
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ISHIKAWA Katsuyuki
Laboratory of Veterinary Pathology, Department of Veterinary Medicine, Faculty of Applied Biological
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HOSOI Masayo
Laboratory of Veterinary Pathology, Department of Veterinary Medicine, Faculty of Applied Biological
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OHTA George
Laboratory of Veterinary Pathology, Department of Veterinary Medicine, Faculty of Applied Biological
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Makino Toshihiko
Medicinal Safety Research Laboratories Daiichi Sankyo Co. Ltd.:department Of Veterinary Pathology Gr
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Hosoi Masayo
Laboratory Of Veterinary Pathology Department Of Veterinary Medicine Faculty Of Applied Biological S
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Mizoshita Tsutomu
Department Of Gastroenterology And Metabolism Nagoya City University Graduate School Of Medical Scie
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Sakai Hiroki
Laboratory Of Biological Chemistry Department Of Chemistry Faculty Of Science Hokkaido University
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Ohta George
Department of Veterinary Pathology, Faculty of Applied Biological Science, Gifu University
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Totsuka Yukari
国立がん研究センター研究所 発がんシステム研究分野
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Ishikawa Katsuyuki
Laboratory Of Veterinary Pathology Department Of Veterinary Medicine Faculty Of Applied Biological S
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Makita Takashi
Dept. Vet. Anat. Yamaguchi Univ.
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Makita Takashi
Medicinal Safety Research Laboratories Daiichi Sankyo Co. Ltd.:department Of Veterinary Pathology Gr
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YANAI Tokuma
Laboratory of Veterinary Pathology, Department of Veterinary Medicine, Faculty of Applied Biological Sciences, Gifu University
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