Application of 4-Iodophenol-enhanced Luminol Chemiluminescence to Direct Detection of Horseradish Peroxidase Encapsulated in Liposomes

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概要

• 論文の詳細を見る

• 4-Iodophenol was applied to an enhancer in the direct detection of horseradish peroxidase (HRP) encapsulated in liposomes by using luminol chemiluminescence (CL). Luminol, 4-iodophenol and hydrogen peroxide permeate into the inner phase of liposomes containing HRP, resulting in the progress of 4-iodophenol-enhanced luminol CL catalyzed by HRP in liposomes. The CL intensity observed in liposomes was a factor of 150 greater than that observed in a lipid-free bulk solution. The detection limit in the direct detection of HRP encapsulated in liposomes was sensitive by a factor of 30 compared with that in a lipid-free bulk solution. 4-Iodophenol effectively functioned as an enhancer in HRP-catalyzed luminol CL in liposomes.

• 2009-09-10

著者

• KAMIDATE Tamio

  Division of Biotechnology and Molecular Chemistry, Graduate School of Engineering, Hokkaido Universi

• MARUYA Masumi

  Division of Biotechnology and Molecular Chemistry, Graduate School of Engineering, Hokkaido Universi

• TANI Hirofumi

  Division of Biotechnology and Molecular Chemistry, Graduate School of Engineering, Hokkaido Universi

• ISHIDA Akihiko

  Division of Biotechnology and Molecular Chemistry, Graduate School of Engineering, Hokkaido Universi

• Ishida Akihiko

  Division Of Biotechnology And Molecular Chemistry Graduate School Of Engineering Hokkaido University

• Maruya Masumi

  Division Of Biotechnology And Molecular Chemistry Graduate School Of Engineering Hokkaido University

• 谷 博文

  Division Of Biotechnology And Molecular Chemistry Graduate School Of Engineering Hokkaido University

• Tani Hirofumi

  Division Of Biotechnology And Macromolecular Chemistry Graduate School Of Engineering Hokkaido Unive

• Kamidate T

  Hokkaido Univ. Sapporo

• Kamidate Tamio

  Division Of Biotechnology And Molecular Chemistry Graduate School Of Engineering Hokkaido University

• Kamidate Tamio

  Laboratory Of Bioanalytical Chemistry Graduate School Of Engineering Hokkaido University

• Kamidate Tamio

  Division Of Biotechnology And Macromolecular Chemistry Graduate School Of Engineering Hokkaido Unive

• Ishida Akihiko

  Division Of Molecular Chemistry Graduate School Of Engineering Hokkaido University

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