Functional interaction of hepatitis C virus NS5B with nucleolin GAR domain
スポンサーリンク
概要
- 論文の詳細を見る
Hepatitis C Virus (HCV) non-structural proteins are major components of replication complex that is modulated by several host factors. We previously reported that nucleolin, a representative nucleolar marker, interacts with the NS5B through two separated sequences, amino acids (aa) 208-214 and 500-506, and that W208 in the former stretch is essential for both nucleolin-binding and HCV replication. Here we evaluated the role of the latter stretch aa 500-506 of WRHRARS in nucleolin-binding and HCV replication scanned by alanine-substituted clustered mutant (cm) or point mutant (pm). One tryptophan and three arginine residues in the sequence were found to be essential both for nucleolin-binding in vivo and HCV replication detected with a HCV subgenomic replicon transfected into Huh7 cells. NS5B-binding of nucleolin was further delineated by truncation and clustered mutants of nucleolin. Arginine-glycine-glycine (RGG) repeat in the Glycine arginine rich (GAR) domain were defined to be indispensable for NS5B-binding immunologically detected in in vivo and in vitro although short internal-truncations of RGG repeat are tolerable for NS5B-binding. These results indicate that nucleolin is a critical host factor for HCV replication through the direct interaction between W208 and several residues at the sequence, aa 500-505, of NS5B, and the long-turn motif including RGG repeat at nucleolin C-terminal. © 2007 The Japanese Biochemical Society.
- 日本生化学会 = Japanese Biochemical Societyの論文
- 2007-06-01
著者
-
Kaneko Shuichi
Department Of Disease Control And Homeostasis Kanazawa University Graduate School Of Medical Science
-
Yoshioka Katsuji
Division Of Molecular Cell Signaling Department Of Molecular And Cellular Biology Cancer Research In
-
MURAKAMI Seishi
Division of Molecular Cell Signaling, Department of Molecular and Cellular Biology, Cancer Research
-
Kaneko Shuichi
Department Of Cancer Gene Regulation Kanazawa University
-
Kaneko Shuichi
Department Of Gastroenterology University Hospital Kanazawa University
-
KUSAKAWA Takashi
Division of Signal Transduction, Cancer Research Institute, Kanazawa University
-
SHIMAKAMI Tetsuro
Department of Gastroenterology, University Hospital, Kanazawa University
-
Murakami Seishi
Division Of Molecular Cell Signaling Department Of Molecular And Cellular Biology Cancer Research In
-
Murakami Seishi
Division Of Signal Transduction Cancer Research Institute Kanazawa University
-
Kusakawa Takashi
Division Of Signal Transduction Cancer Research Institute Kanazawa University
-
Shimakami Tetsuro
Department Of Gastroenterology University Hospital Kanazawa University
-
Yoshioka Katsuji
Division Of Signal Transduction Cancer Research Institute Kanazawa University
-
Yoshioka Katsuji
Division Of Cell Cycle Regulation Department Of Molecular And Cellular Biology Cancer Research Insti
関連論文
- Reactive peripheral blood plasmacytosis in a patient with acute hepatitis A
- MafB protein stability is regulated by the JNK and ubiquitin-proteasome pathways
- Hepatitis B virus X protein overcomes oncogenic RAS-induced senescence in human immortalized cells
- Virological effects and safety of combined double filtration plasmapheresis (DFPP) and interferon therapy in patients with chronic hepatitis C: A preliminary study
- Case-control study for the identification of virological factors associated with fulminant hepatitis B
- Timing of interferon therapy and sources of infection in patients with acute hepatitis C
- The efficacy of ursodeoxycholic acid and bezafibrate combination therapy for primary biliary cirrhosis : A prospective, multicenter study
- The committee for revision of the Clinical Practice Guidelines for Hepatocellular Carcinoma
- Liver steatosis, but not fibrosis, is associated with insulin resistance in nonalcoholic fatty liver disease
- Apoptosis of murine cultured biliary epithelial cells induced by glycochenodeoxycholic acid involves Fas receptor and its ligand
- Selective estrogen receptor modulator raloxifene-associated aggravation of nonalcoholic steatohepatitis.
- Transformation of p53-positive papillary thyroid carcinoma to anaplastic carcinoma of the liver following postoperative radioactive iodine-131 therapy
- LIVING-RELATED LIVER TRANSPLANTATION FOR PEDIATRIC PATIENTS(International Symposium ,第29回日本小児外科学会総会)
- DPE-068 Muscle Atrophy F-box Inhibited Pressure Overload Induced Cardiac Hypertrophy through the Activation of NF κ-B Pathway(DPE12,Cardiomyopathy/Hypertrophy (Basic) (M),Digital Poster Session (English),The 73rd Annual Scientific Meeting of The Japanese
- PJ-672 G_ and G_q Coordinately Mediate S1P_2-induced Inhibition of Rac and Migration in Vascular Smooth Muscle in Rho-dependent Manner(PJ113,Atherosclerosis, Basic 2 (IHD),Poster Session (Japanese),The 73rd Annual Scientific Meeting of The Japanese
- PJ-373 Sphingosine-1-Phosphate Inhibits the Formation of Tube-Like Structures via Its Receptor EdgS in Vascular Endothelial Cells(Angiogenesis 3 (IHD) : PJ62)(Poster Session (Japanese))
- OE-012 Knockdown of Edg1 Gene Expression by RNA Interference Abolishes Sphingosine-1-Phosphate-Induced Platelet-Derived Growth Factor-B Chain Expression in Vascular Smooth Muscle Cells(Vasoactive Compound/Growth Factor/Cytokine 1 (H) : OE2)(Oral Presentat
- Current Status of Anticoagulation Therapy for Elderly Atrial Fibrillation Patients in Japan : From Hokuriku Atrial Fibrillation Trial
- PJ-232 Alterd Interaction between Plasminogen Activator Inhibitor Type 1 Activity and Sympathetic Nerve Activity with Aging(Atherosclerosis,clinical(10)(IHD),Poster Session(Japanese),The 72nd Annual Scientific Meeting of the Japanese Circulation Society)
- PE-328 Present Educational System of Resuscitation dose not Encourage High School Students to fight Cardiopulmonary Arrest(Emergency care/CPR(03)(H),Poster Session(English),The 72nd Annual Scientific Meeting of the Japanese Circulation Society)
- PE-146 Current Status of Anticoagulation Therapy for Elderly Patients, from Hokuriku Atrial Fibrillation Trial(Arrhythmia, therapy(12)(A),Poster Session(English),The 72nd Annual Scientific Meeting of the Japanese Circulation Society)
- PE-124 Antioxidative Effect Provided with Statin Is Independent from LDL Lowering Mechanisms in the Clinical Course of Acute Coronary Syndrome(Acute coronary syndrome, basic/clinical(03)(IHD),Poster Session(English),The 72nd Annual Scientific Meeting of t
- PE-033 Altered Firing Pattern of Single-unit Sympathetic Vasoconstrictor Fibers during Exercise in Chronic Heart Failure(Autonomic nervous system(03)(H),Poster Session(English),The 72nd Annual Scientific Meeting of the Japanese Circulation Society)
- OE-102 Sympathetic Augmentation was Induced by Strict Rate Control in AF Patient(Autonomic nervous system(01)(H),Oral Presentation(English),The 72nd Annual Scientific Meeting of the Japanese Circulation Society)
- Altered Interaction Between Plasminogen Activator Inhibitor Type 1 Activity and Sympathetic Nerve Activity With Aging
- PJ-467 Interaction of Low Dose of Endotheline Blockade and Beta-adrenergic Receptor Antagonist Improved Coronary Flow Reserve after Ischemia Reperfusion Injury(Coronary circulation, basic/clinical-4, The 71st Annual Scientific Meeting of the Japanese Circ
- PJ-263 Additional Administration of Angiotensin Receptor Blockade with Aldosteron Antagonist Improved Oxidative Stress after Acute Coronary Syndrome in Human(Myocardial ischemia/reperfusion, basic/clinical-5, The 71st Annual Scientific Meeting of the Japa
- PJ-260 The Reduction of Cellular ER Stress Regarding with Chemical Chaperon Improves Myocardial Ischemia Reperfusion Injury in Mice(Myocardial ischemia/reperfusion, basic/clinical-5, The 71st Annual Scientific Meeting of the Japanese Circulation Society)
- PJ-077 Atrial Fibrillation Itself Augments Single-unit Muscle Sympahtetic Nerve Activity in Congestive Heart Failure(Autonomic nervous system-3, The 71st Annual Scientific Meeting of the Japanese Circulation Society)
- PE-203 Effect of Elevated Arterial Pressure on Vascular Functional and Structural Atherosclerosis in the Hypertensive Patients(Atherosclerosis, clinical-05, The 71st Annual Scientific Meeting of the Japanese Circulation Society)
- PE-137 Azelnidipine Inhibits Single-unit Muscle Sympathetic Nerve Activity in Healthy Humans(Autonomic nervous system-2, The 71st Annual Scientific Meeting of the Japanese Circulation Society)
- PJ-576 Interaction of Endotheline Blockade and Beta-adrenergic Receptor Antagonist Improved Myocardial Apoptosis after Ischemia Reperfusion Injury in the Rat Heart(Acute coronary syndrome, basic/clinical-8 (IHD) PJ97,Poster Session (Japanese),The 70th Ann
- Chemokine/chemokine receptor-mediated inflammation regulates pathologic changes from acute kidney injury to chronic kidney disease
- Human telomerase exists in two distinct active complexes in vivo
- The Scaffold Protein c-Jun NH2-Terminal Kinase-associated Leucine Zipper Protein Regulates Cell Migration through Interaction with the G Protein G13
- Regulation of N-cadherin-based cell–cell interaction by JSAP1
- Graves Disease Associated with Infectious Mononucleosis due to Primary Epstein-Barr Virus Infection: Report of 3 Cases
- Involvement of IL-10, an anti-inflammatory cytokine in murine liver injury induced by Concanavalin A
- The Rho/Rho-kinase Systems Are Involved in Rapid Pacing-induced Changes of Atrial Refractory Period in a Canine Model
- Quantiative Analysis of Oxidative Stress Directly Reflect the Clinical Course of Acute Coronary Syndrome(Acute Coronary Syndrome, Basic/Clinical 5 (IHD), The 69th Annual Scientific Meeting of the Japanese Circulation Society)
- Interaction of Endotheline Blockade and Aldosterone Blockade Improved Myocardial Dysfunction After Ischemia Reperfusion Injury in the Rat Heart(Acute Myocardial Infarction, Basic 4 (IHD), The 69th Annual Scientific Meeting of the Japanese Circulation Soci
- PJ-019 A New System for the Continuous Automated Measurement of the Carotid Arterial Wall Dynamics(Echo/Doppler 2 (I) : PJ4)(Poster Session (Japanese))
- PE-093 Influences of Pressor Stimuli on the Distensibility of Carotid Artery in Humans(Autonomic Nervous System 1 (H) : PE16)(Poster Session (English))
- PE-003 Rho-kinase Inhibitor Fasudil Prevents Atrial Electrical Remodeling in Atrial Fibrillation(Arrhythmia, Basic 2 (A) : PE1)(Poster Session (English))
- OJ-200 Effect of Aging on Low Frequency Components of Peripheral Vascular Resistance Variability in Human(Autonomic Nervous System 2 (H) : OJ23)(Oral Presentation (Japanese))
- Subacute Thyroiditis Developed in Identical Twins Two Years Apart
- Fibrocyte : New participant in the pathogenesis of renal fibrosis
- The role of fibrocytes in progressive renal fibrosis
- Tiny staining spots in liver cirrhosis associated with HCV infection observed by computed tomographic hepatic arteriography: follow-up study
- Relationship between Plasma hANP Level and Pretibial Edema by Pioglitazone Treatment
- An open-label study of administration of EH0202, a health-food additive, to patients with chronic hepatitis C
- Development of evidence-based clinical guidelines for the diagnosis and treatment of hepatocellular carcinoma in Japan
- Molecular functions and biological roles of hepatitis B virus x protein
- Optimal amount of monocyte chemoattractant protein-1 enhances antitumor effects of suicide gene therapy against hepatocellular carcinoma by M1 macrophage activation
- PE-002 Additional Administration of T-type Ca Antagonist Significantly Improved Oxidative Stress After Acute Coronary Syndrome in Human(Acute coronary syndrome, basic/clinical-4 (IHD) PE1,Poster Session (English),The 70th Anniversary Annual Scientific Mee
- Molecular cloning, and characterization and expression of dihydrolipoamide acetyltransferase component of murine pyruvate dehydrogenase complex in bile duct cancer cells
- Functional interaction of hepatitis C virus NS5B with nucleolin GAR domain
- Involvement of CD11b+ GR-1low cells in autoimmune disorder in MRL-Faslpr mouse
- A case of neurosarcoidosis with necrotizing granuloma expressing angiotensin-converting enzyme
- Living-related liver transplantation : Report of experiences at Shinshu University Hospital
- Phase I/II study of the pharmacokinetics, safety and efficacy of S-1 in patients with advanced hepatocellular carcinoma
- S-I-2 Living-related liver transplantation (LRLT) for pediatric patients
- Involvement of bone-marrow-derived cells in kidney fibrosis
- Scaffold proteins in mammalian MAP kinase cascades
- Clinical impact of albuminuria in diabetic nephropathy
- Benidipine reduces ischemia reperfusion-induced systemic oxidative stress through suppression of aldosterone production in mice
- Acute Hemodynamic Effect of Cardiac Resynchronization Therapy Derived from Phase Analysis on Gated Myocardial Perfusion Spect
- Noncontact Mapping-Guided Sinus Node Modification for Inappropriate Sinus Tachycardia
- Slowly Progressive Insulin-Dependent Diabetes in a Patient with Primary Biliary Cirrhosis with Portal Hypertension-Type Progression
- Synthesis, Structure, and Physiological Effects of Peroxovanadium(V) Complexes Containing Amino Acid Derivatives as Ancillary Ligands
- Magnetic resonance imaging in a patient with an implantable cardiac defibrillator
- Duodenal adenocarcinoma with neuroendocrine features in a patient with acromegaly and thyroid papillary adenocarcinoma : a unique combination of endocrine neoplasia
- Fatty liver as a consequence and cause of insulin resistance : Lessons from type 2 diabetic liver
- Three cases of pneumatosis intestinalis presenting in autoimmune diseases
- Abnormal liver function tests and metabolic syndrome - Is fatty liver related to risks for atherosclerosis beyond obesity?
- Effects of adipose-derived mesenchymal cells on ischemia-reperfusion injury in kidney
- Successful Delivery in a Patient with Antineutrophil Cytoplasmic Antibody-associated Glomerulonephritis
- Clinical Characteristics of Patients who Developed Hepatocellular Carcinoma after Hepatitis C Virus Eradication with Interferon Therapy: Current Status in Japan
- Intra-thyroid blood flow in Plummer's disease.
- Guideline on the use of new anticancer drugs for the treatment of Hepatocellular Carcinoma 2010 update
- Angiotensin II type 1 receptor blocker attenuates diabetes-induced atrial structural remodeling
- Fractalkine and its receptor, CX3CR1, promote hypertensive interstitial fibrosis in the kidney
- Nodular lesions and mesangiolysis in diabetic nephropathy