Release of Endogenous Glutamate by AMPA Receptors Expressed in Cultured Rat Costal Chondrocytes(Pharmacology)
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概要
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We have previously demonstrated the release of endogenous glutamate by activation of DL-α-amino-3-hydroxy-5-methylisoxasole-4-propionate (AMPA) receptors expressed by bone, while there is no information available on the possible functional expression of glutamatergic signaling molecules in cartilage to date. In rat costal chondrocytes cultured for 4 to 28d, expression of mRNA was seen for several chondral marker genes including sox9, runt-related gene 2/core binding factor α-1 (Runx-2/Cbfa-1), type II collagen and aggrecan, but not for the adipocyte marker gene peroxisome proliferator-activated receptor γ (PPARγ). Expression of mRNA was drastically increased for Runx-2/Cbfa-1 during culturing from 7 to 14 d with a gradual increase thereafter up to 28d, while a transient increase was seen in mRNA expression for both type-II collagen and sox-9 on 14 d and for aggrecan on 7 d respectively, in chondrocytes cultured for a period up to 28d. Irrespective of the culture period up to 21d, marked expression was seen by cultured chondrocytes with mRNA for GluR3 subunit of AMPA receptors, in addition to vesicular glutamate transporter-1 (VGLUT1) required for the condensation and subsequent exocytotic release of glutamate in the glutamatergic neurotransmission in the brain. Cultured rat costal chondrocytes underwent spontaneous release of endogenous glutamate, while an inhibitor of AMPA receptor desensitization significantly prolonged the duration of endogenous glutamate release stimulated by AMPA. These results suggest that endogenous glutamate could be released from intracellular vesicular constituents associated with VGLUT1 through activation of AMPA receptors expressed by cultured rat costal chondrocytes.
- 公益社団法人日本薬学会の論文
- 2005-06-01
著者
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HINOI Eiichi
Laboratory of Molecular Pharmacology, Division of Pharmaceutical Sciences, Kanazawa University Gradu
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YONEDA Yukio
Laboratory of Molecular Pharmacology, Division of Pharmaceutical Sciences, Kanazawa University Gradu
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Yoneda Yukio
Lab. Of Molecular Pharmacology Div. Of Pharmaceutical Sciences Kanazawa Univ. Graduate School Of Nat
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Yoneda Yukio
Laboratory Of Molecular Pharmacology Division Of Pharmaceutical Sciences Kanazawa University Graduat
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Hinoi Eiichi
Lab. Of Molecular Pharmacology Div. Of Pharmaceutical Sciences Kanazawa Univ. Graduate School Of Nat
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Hinoi Eiichi
Laboratory Of Molecular Pharmacology Division Of Pharmaceutical Sciences Kanazawa University Graduat
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Wang Liyang
Laboratory of Molecular Pharmacology, Division of Pharmaceutical Sciences, Kanazawa University Gradu
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TAKEMORI Akihiro
Laboratory of Molecular Pharmacology, Division of Pharmaceutical Sciences, Kanazawa University Gradu
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Wang Liyang
Laboratory Of Molecular Pharmacology Division Of Pharmaceutical Sciences Kanazawa University Graduat
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Takemori Akihiro
Laboratory Of Molecular Pharmacology Division Of Pharmaceutical Sciences Kanazawa University Graduat
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