Determination of Honokiol and Magnolol by Micro HPLC with Electrochemical Detection and Its Application to the Distribution Analysis in Branches and Leaves of Magnolia obovata
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概要
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A simple and sensitive method has been developed for determining honokiol and magnolol in fresh Magnolia obovata (M. obovata) by micro high-performance liquid chromatography with electrochemical detection (μHPLC-ECD). Chromatography was performed using a Capcell Pak C-18 UG 120 microbore octadecylsilica (ODS) column, methanol-water-phosphoric acid (65 : 35 : 0.5, v/v/v), as a mobile phase and applied potential at+0.8V vs. Ag/AgCl. Peak heights were found linearly related to the amounts of honokiol and magnolol injected from 0.67pg to 2.0ng (r>0.999). The detection limits (S/N=3) were 0.13pg, respectively. Honokiol and magnolol of 0.27ng were detected with relative standard deviation (RSD) of 0.73 and 1.17% (n=5), respectively. Honokiol and magnolol in Magnolia Bark of the Japanese Pharmacopoeia were extracted with 70% methanol, diluted with a mobile phase, and injected into the μHPLC-ECD for determination. Recoveries of honokiol and magnolol in Magnolia Bark exceeded 98.7% with RSD, less than 0.93% (n=5). Determination of the distributions of honokiol and magnolol in bark, phloem, wood, leaf blades, and petioles of fresh M. obovata were made using weight samples of 40-238mg. This method is useful to determine honokiol and magnolol in M. obovata, which is a candidate for crude magnolia bark for traditional Japanese herbal medicines.
- 公益社団法人日本薬学会の論文
- 2005-03-01
著者
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小谷 明
Department Of Analytical Chemistry School Of Pharmacy Tokyo University Of Pharmacy And Life Sciences
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Kojima Satoshi
Department Of Cardiology Juntendo Shizuoka Hospital
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小谷 明
School Of Pharmacy Tokyo Univ. Of Pharmacy And Life Sciences
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Kojima Satoshi
School Of Pharmacy Tokyo University Of Pharmacy And Life Sciences
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HAKAMATA Hideki
Department of Biochemistry Kumamoto University School of Medicine
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KOTANI Akira
Department of Analytical Chemistry, School of Pharmacy, Tokyo University of Pharmacy and Life Scienc
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KUSU Fumiyo
Department of Analytical Chemistry, School of Pharmacy, Tokyo University of Pharmacy and Life Scienc
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JIN Dongri
Chemistry Department of Science and Engineering College, Yanbian University
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Kusu F
School Of Pharmacy Tokyo University Of Pharmacy And Life Sciences
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Kusu Fumiyo
School Of Pharmacy Tokyo Univ. Of Pharmacy And Life Sciences
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Jin D
Chemistry Department Of Science & Engineering College Yanbian University
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袴田 秀樹
School Of Pharmacy Tokyo University Of Pharmacy And Life Sciences
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Hakamata Hideki
Department Of Biochemistry And Department Of Metabolic Medicine Kumamoto University School Of Medici
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Kojima Satoshi
Department Bf Physics Faculty Of Science And Engineering Ritsumeikan University
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Hakamata Hideki
Department Of Analytical Chemistry School Of Pharmacy Tokyo University Of Pharmacy And Life Sciences
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Kotani A
School Of Pharmacy Tokyo University Of Pharmacy And Life Science
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Kusu Fumiyo
Department Of Analytical Chemistry School Of Pharmacy Tokyo University Of Pharmacy And Life Sciences
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