Structure-activity analysis of an antimicrobial peptide derived from bovine apolipoprotein A-II
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概要
- 論文の詳細を見る
We previously showed that bovine apolipoprotein A-II (apoA-II) has antimicrobial activity against Escherichia coli in PBS, and its C-terminal residues 49-76 are responsible for the activity using synthetic peptides. In order to understand the structural requirements of peptide 49-76 for the antimicrobial activity, the N- or C-terminus was trun-cated and then the charged (Lys or Asp) or Ser residues were replaced by Ala. Deletion of the first or last three amino acids and replacement of Lys-54155 or 71/72 by Ala caused a substantial decreases in α-helical content in 50% TFE, showing the possible presence of helices in N- and C-terminal regions, respectively. The anti-Escherichia coli activity of the peptide correlated with its liposome-binding activity. Replacement of Lys-54/55 or 71/72 by Ala resulted in an almost complete loss of anti-E. con activity with a substantial decrease in liposome-binding activity. Moreover, deletion of the last three amino acids caused a reduction to 1/17 of the original anti-E. coli activity with a moderate decrease in liposome-binding activity. In contrast, replacement of Ser-65/66, Asp-59, or Asp-69 by Ma hardly affected the anti-E. coli activity. These findings suggest that Lys-54/55 and Lys-71/72 on the putative helices are critical for antimicrobial activity, and the C-terminal 3 amino acids are important for the structural integrity of the C-terminal region for effective antimicrobial activity.
- 社団法人 日本生化学会の論文
- 2002-07-01
著者
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SATOH Takanori
Department of Chemistry, College of Science and Engineering, Aoyama Gakuin University
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Samejima T
Department Of Chemistry College Of Science And Engineering Aoyamagakuin University
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Tsurugi K
Departments Of Biochemistry 2 Yamanashi Medical University
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Kojima S
Institute For Biomolecular Science Gakushuin University
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Motizuki Mitsuyoshi
Department Of Biochemistry 2 Faculty Of Medicine University Of Yamanashi
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Miura K
Institute For Biomolecular Science Faculty Of Science Gakushuin University
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Samejima T
Department Of Acoustic Design Kyushu Institute Of Design
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Yokota S
Biology Laboratory Interdisciplinary Graduate School Of Medicine And Engineering University Of Yaman
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Itoh T
Morinaga Milk Ind. Co. Ltd. Kanagawa
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Takei Toshiaki
Institute For Biomolecular Science Gakushuin University
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Satoh Takanori
Department of Chemistry, College of Science and Engineering, Aoyama Gahuin University
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