Identification of alien chromosomes in a series of Allium fistulosum - A. cepa monosomic addition lines by means of genomic in situ hybridization

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概要

• 論文の詳細を見る

• Genomic in situ hybridization (GISH) was used to identify alien chromosomes in a series of eight different Allium fistulosum - A. cepa monosomic addition lines. Biotin-labeled total genomic DNA from shallot (A. cepa L. Aggregatum group) was used as a probe together with an excess amount of unlabeled blocking DNA from the recipient plant, Japanese bunching onion (A. fistulosum L.). Probe hybridization sites were detected by FITC-conjugated avidin and anti-avidin antibody using an epifluorescence microscope. In each mitotic metaphase cell of all the eight types of monosomic addition lines, the alien chromosomes were successfully discriminated from other 16 A. fistulosum chromosomes. Furthermore, no clear exchanges of chromosome segments between A. cepa and A. fistulosum were observed. This finding indicates that in each addition line an entire (unrecombined) A. cepa chromosome is present in an integral diploid background of A. fistulosum.

• 日本遺伝学会の論文

著者

• Tashiro Y

  Saga Univ. Saga Jpn

• Tashiro Yosuke

  Department Of Biotechnology And Plant Breeding Faculty Of Agriculture Saga University

• SHIGYO Masayoshi

  Department of Biological and Environmental Sciences, Faculty of Agriculture, Yamaguchi University

• Tashiro Y

  Department Of Biotechnology And Plant Breeding Faculty Of Agriculture Saga University

• Imamura Kenzi

  Department Of Biotechnology And Plant Breeding Faculty Of Agriculture Saga University

• IINO Mitsuyasu

  Department of Biotechnology and Plant Breeding, Faculty of Agriculture, Saga University

• YAMASHITA Ken-ichiro

  Department of Biotechnology and Plant Breeding, Faculty of Agriculture, Saga University

• Iino Mitsuyasu

  Department Of Biotechnology And Plant Breeding Faculty Of Agriculture Saga University

• Yamashita Ken-ichiro

  Department Of Biotechnology And Plant Breeding Faculty Of Agriculture Saga University

• Shigyo Masayoshi

  Department Of Biological And Environmental Sciences Faculty Of Agriculture Yamaguchi University

関連論文

• Complete assignment of structural genes involved in flavonoid biosynthesis influencing bulb color to individual chromosomes of the shallot (Allium cepa L.)
• Molecular and biochemical identification of alien chromosome additions in shallot (Allium cepa L.. Aggregatum group) carrying extra chromosome(s) of bunching onion (A. fistulosum L.)
• Effect of single alien chromosome from shallot (Allium cepa L. Aggregatum group) on carbohydrate production in leaf blade of bunching onion (A. fistulosum L.)
• Production and characterization of alien chromosome additions in shallot (Allium cepa L. Aggregatum group) carrying extra chromosome(s) of Japanese bunching onion (A. fistulosum L.)
• Single alien chromosome additions from shallot (Allium cepa L. Aggregatum group) increase endogenous polyphenol contents in Japanese bunching onion
• Chromosomal locations of genes related to flavonoid and anthocyanin production in leaf sheath of shallot (Allium cepa L. Aggregatum group)
• Identification of alien chromosomes in a series of Allium fistulosum - A. cepa monosomic addition lines by means of genomic in situ hybridization
• Assignment of randomly amplified polymorphic DNA markers to all chromosomes of shallot(Allium cepa L. Aggregatum group)
• Morphological characteristics of a series of alien monosomic addition lines of Japanese bunching onion (Allium fistulosum L.) with extra chromosomes from shallot (A. cepa L. Aggregatum group)
• Establishment of a series of alien monosomic addition lines of Japanese bunching onion (Allium fistulosum L.) with extra chromosomes from shallot (A. cepa L. Aggregatum group)
• Biochemical and Genetic Analysis of Carbohydrate Accumulation in Allium cepa L.
• Development of microsatellite markers in cultivated and wild species of sections Cepa and Phyllodolon in Allium
• ネギ(Allium fistulosum L.)及びシャロット(A.cepa L.Aggregatum group)におけるグルタミン酸-オキサロ酢酸トランスアミナ-ゼ遺伝子の座乗染色体の決定〔英文〕
• Fertility of alien monosomic addition lines of Japanese bunching onion (Allium fistulosum L.) with extra chromosomes from shallot (A. cepa L. Aggregatum group)
• Single alien chromosome additions from shallot (Allium cepa L. aggregatum group) increase endogenous polyphenol contents in Japanese bunching onion
• Biochemical Analyses of the Antioxidative Activity and Chemical Ingredients in Eight Different Allium Alien Monosomic Addition Lines

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