On-Chip Single-Cell Lysis for Extracting Intracellular Material
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概要
- 論文の詳細を見る
A newly designed microfluidic chip with a pinched-channel structure and two pairs of electrodes has been developed to enable easier single-cell capture and lysis. The function of the chip was evaluated by introducing zucchini protoplast cells into the channel. In the first experiment, we attempted to break a cell using the through force of a triangular pinched structure via electroosmotic flow generated by outer electrodes. The pinched structure appeared to break the cell without applying the electric field to the cell directly; however, in this case, the breakable size of the cell was limited by the width of the pinched structure. The next attempt was to break cells regardless of their sizes using a pair of inner electrodes located under the pinched structure. The inner electrodes generated a gradient electric field around the captured cell by applying an alternative voltage to the electrodes. Captured cells with a diameter from 40 to 85 μm could be broken using the inner electrodes with a trapezoidal pinched structure, and the cells were successfully broken at 10 Vpp or less at a frequency of 1 MHz.
- Published by the Japan Society of Applied Physics through the Institute of Pure and Applied Physicsの論文
- 2007-09-30
著者
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Yanagida Yasuko
Precision And Intelligence Laboratory Tokyo Institute Of Technology
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Hatsuzawa Takeshi
Precision And Intelligence Laboratory Tokyo Institute Of Technology
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Tanaka Nobuaki
Mechatronics Technology Development Center, NSK Ltd., Fujisawa, Kanagawa 251-8501, Japan
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Hatsuzawa Takeshi
Precision and Intelligence Laboratory, Tokyo Institute of Technology, Yokohama 226-8503, Japan
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Ikeda Norifumi
Mechatronics Technology Development Center, NSK Ltd., Fujisawa, Kanagawa 251-8501, Japan
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- On-Chip Single-Cell Lysis for Extracting Intracellular Material