Preparation of Fluorescent Poly(methyl methacrylate) Beads Hybridized with Y3Al5O12:Ce3+ Nanophosphor for Biological Application
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概要
- 論文の詳細を見る
Poly(methyl methacrylate) beads were hybridized with Y3Al5O12:Ce3+ nanoparticles through electrostatic interactions using the layer-by-layer adsorption technique to prepare fluorescent beads for biological application. The subsequent sequential adsorption of poly(sodium 4-styrenesulfonate) and poly(allylamine hydrochloride) onto the composite beads prevented the detachment of the Y3Al5O12:Ce3+ nanoparticles. The fluorescent beads were observed as tight dot plots by flowcytometry. Bovine serum albumin can be immobilized on the surface of the composite beads and tagged with a red dye through an antigen–antibody reaction. This result indicates that the composite beads are useful for bead assays such as flowcytometry.
- Published by the Japan Society of Applied Physics through the Institute of Pure and Applied Physicsの論文
- 2007-08-15
著者
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Isobe Tetsuhiko
Department Of Applied Chemistry Faculty Of Science And Technology Keio University
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OHKUBO Michio
Yokohama R & D Lab. The Furukawa Electric Co., Ltd.
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Kurokawa Kiyoshi
Department Of Anatomy School Of Medicine
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Saito Daisuke
Department of Applied Chemistry, Faculty of Science and Technology, Keio University, 3-14-1 Hiyoshi, Kohoku-ku, Yokohama 223-8522, Japan
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Asakura Ryo
Department of Applied Chemistry, Faculty of Science and Technology, Keio University, 3-14-1 Hiyoshi, Kohoku-ku, Yokohama 223-8522, Japan
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Kusayama Ikumi
Department of Applied Chemistry, Faculty of Science and Technology, Keio University, 3-14-1 Hiyoshi, Kohoku-ku, Yokohama 223-8522, Japan
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Hirayama Yosuke
Yokohama R&D Laboratories, Furukawa Electric Co., Ltd., 2-4-3 Okano, Nishi-ku, Yokohama 220-0073, Japan
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Aizawa Hideki
Yokohama R&D Laboratories, Furukawa Electric Co., Ltd., 2-4-3 Okano, Nishi-ku, Yokohama 220-0073, Japan
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Takagi Tomohiro
Yokohama R&D Laboratories, Furukawa Electric Co., Ltd., 2-4-3 Okano, Nishi-ku, Yokohama 220-0073, Japan
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Aizawa Hideki
Yokohama R&D Laboratories, Furukawa Electric Co., Ltd., 2-4-3 Okano, Nishi-ku, Yokohama 220-0073, Japan
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Takagi Tomohiro
Yokohama R&D Laboratories, Furukawa Electric Co., Ltd., 2-4-3 Okano, Nishi-ku, Yokohama 220-0073, Japan
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Hirayama Yosuke
Yokohama R&D Laboratories, Furukawa Electric Co., Ltd., 2-4-3 Okano, Nishi-ku, Yokohama 220-0073, Japan
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