Biological Characteristics of Laryngeal Leukoplakia

概要

論文の詳細を見る
Laryngeal Leukoplakia lesions often have complicated morphological characteristics. The degree of DNA-instability as revealed by an immunohistochemical staining with anti-single-stranded DNA antibody after acid hydrolysis (DNA-instability test) was used as a marker of malignancy (Fukuda et al, 1986, 1993). The cases to which this was applied are as follows : 8 laryngeal leukoplakia, 14 oral leukoplakia, 19 squamous cell carcinoma, and 12 normal squamous epithelium. Ordinary paraffin sections taken from each of the cases were hydrolyzed for the denaturation of unstable DNA, and successively stained with anti-single-stranded DNA antiserum immunohistochemistry. The level of proliferative activity and the polarity of the proliferative cell distribution were evaluated by PCNA-immunohistochemistry. Quantitative analyses of the number, mean and largest size, and maximum shape-irregularity evaluated by AgNORs staining in the nucleus were performed. Eight laryngeal leukoplakia and 12 oral leukoplakia cases with dysplasia (90%) were positively and diffusely stained by the DNA-instability test indicating their malignancy. Reflecting their malignant character, these cases showed a remarkable increase in PCNA-index with the loss of polarity of PCNA-positive cell distribution, and also increased maximum shape-irregularity of AgNORs. In conclusion, the majority of leukoplakia occurrences should be regarded the same as in-situ cancer.
The Japan Laryngological Associationの論文

Biological Characteristics of Laryngeal Leukoplakia

スポンサーリンク

概要

著者

関連論文

スポンサーリンク