Induction of CD44 and MMP Expression by Hyaluronidase Treatment of Articular Chondrocytes
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概要
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In this study, the effects of fragmentation of the glycosoaminoglycans of the cell-associated matrix by hyaluronidase (HAase) on the expression of CD44 receptor and matrix metalloproteinase (MMP) mRNAs in cultured articular chondrocytes were examined. Chondrocytes, isolated from rabbit and bovine articular cartilage, were treated with bovine testicular HAase (0-200 units/ml) in the presence or absence of an antibody for CD44. The mRNA levels of CD44, CD44 variant (CD44v), MMPs (MMP-1, -3 and -9), and tissue inhibitors of metalloproteinases (TIMP-1 and TIMP-2) were determined by RT PCR. The treatment of cultured chondrocytes with HAase resulted in the production of low molecular weight fragments of hyaluronan (HA). The expression of CD44, CD44v and MMP (MMP-1, -3 and -9) mRNAs, but not TIMP-1 or TIMP-2 mRNA, was up-regulated in the cultures treated with HAase, whereas this expression was not affected by treatment with purified HA of 1.0×105 Da. Furthermore, the induction of CD44 and MMPs on treatment with HAase was suppressed by an anti-CD44 antibody. The results suggest that the fragmentation of HA may lead to cartilage destruction in terms of the enhanced expression of MMPs as well as the upregulation of CD44.
- 社団法人 日本生化学会の論文
著者
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Suzuki Aya
Department Of Environmental System Science Graduate School Of Science And Technology Shinshu Univers
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Ueki Masashi
Department Of Biology Faculty Of Science Osaka City University
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Nakatani Yuki
Department Of Biological Information Tokyo Institute Of Technology
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Doi Takeyoshi
Department Of Orthodontics And Craniofacial Developmental Biology Hiroshima University Graduate Scho
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Knudson Warren
Department Of Biochemistry Rush Medical College Rush University Medical Center
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Knudson Cheryl
Department Of Biochemistry Rush Medical College Rush University Medical Center
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Yoneno Kiyoshi
Departments Of Orthodontics And Craniofacial Developmental Biology Hiroshima University Graduate Sch
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Tanimoto Kotaro
Department Of Orthodontics And Craniofacial Developmental Biology Hiroshima University Graduate Scho
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Honda Kobun
Department Of Orthodontics And Craniofacial Developmental Biology Hiroshima University Graduate Scho
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Tanaka Nobuaki
Department Of Applied Chemistry Faculty Of Engineering Okayama University
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TANNE Kazuo
Department of Oral Anatomy, Hiroshima University
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Ohno Shigeru
Department of Chemistry, Faculty of Science, The University of Tokyo
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Ohno-Nakahara Maiko
Department of Orthodontics and Craniofacial Developmental Biology, Hiroshima University Graduate School of Biomedical Sciences
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