Identification of Human GATA-2 Gene Distal IS Exon and Its Expression in Hematopoietic Stem Cell Fractions.
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概要
- 論文の詳細を見る
Transcription factor GATA-2 is essential for the proper function of hematopoietic stem cells and progenitors. Two first exons/promoters have been found in the mouse GATA-2 gene, and a distal IS promoter shows activity specific to hematopoietic progenitors and neural tissues. To ascertain whether the two-promoter system is also utilized in the human GATA-2 gene, we isolated and analyzed a P1 phage clone containing this gene. The nucleotide sequence of the human GATA-2 gene 5' flanking region was determined over 10 kbp, and a human IS exon was identified in the locus through sequence comparison analysis with that of the mouse GATA-2 IS exon. RNA blotting and reverse-transcribed PCR analyses identified a transcript that starts from the IS exon in human leukemia-derived cell lines. The IS-originated transcript was also identified in CD34-positive bone marrow and cord blood mononuclear cells, which are recognized as clinically important hematopoietic stem cell-enriched fractions. Phylogenic comparison of the human and mouse GATA-2 gene sequences revealed several regions in the locus that exhibit high sequence similarity. These results demonstrate that the GATA-2 gene regulatory machinery is conserved among vertebrates. The fact that the human IS promoter is active in the hematopoietic stem cell/progenitor fraction may be an important clue for the design of a vector system that can specifically express various genes in hematopoietic stem cells and progenitors.
- 社団法人 日本生化学会の論文
著者
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Akine Yasuyuki
Institute Of Clinical Medicine University Of Tsukuba
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Harigae Hideo
Department Of Biochemistry Tohoku University School Of Medicine
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Yamamoto Masayuki
Institute Of Basic Medical Sciences And Center For Tsukuba Advanced Research Alliance University Of
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Pan Xiaoqing
Institutes of Basic Medical Sciences and Center for Tsukuba Advanced Research Alliance(TARA)
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Minegishi Naoko
Institutes of Basic Medical Sciences and Center for Tsukuba Advanced Research Alliance (TARA)
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Minegishi Masayoshi
Institute of Aging, Cancer and Developmental Biology, Tohoku University
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Yarnagiwa Hironori
Institutes of Basic Medical Sciences and Center for Tsukuba Advanced Research Alliance (TARA)
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