Specific Assay Method for the Activities of Cathepsin L-Type Cysteine Proteinases.
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概要
- 論文の詳細を見る
We have established a new differential assay method for cathepsin L-type proteinases using specific inhibitors, E-64 for all cysteine proteinases, CA-074 for cathepsin B, and PLCPI for cathepsin L-type proteinases with Z-Phe-Arg-MCA as the substrate. The value of cathepsin B calculated by this method did not coincide with value assayed directly in terms of the hydrolysis of Z-Arg-Arg-MCA, a specific substrate for cathepsin B. The activities of cathepsin L-type proteinases, cathepsins B and J in rat liver and kidney were assayed at the same time using this new assay method as a representative example.
- 社団法人 日本生化学会の論文
著者
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Katunuma Nobuhiko
Institute For Enzyme Research Dept. Of Enzyme Chemistry Tokushima Univ.
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KISHINO Yasuo
Department of Nutrition
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Kakegawa Hisao
Institute for Health Sciences, Tokushima Bunri University
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Inubushi Tomoko
Institute for Health Sciences, Tokushima Bunri University
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