MORPHOLOGICAL STUDIES ON ISOLATED RAT ADIPOCYTES II. EFFECTS OF INSULIN, WASHING AND PHOSPHOLIPASES
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概要
- 論文の詳細を見る
The effect of insulin on the surface structure of isolated adipocytes was examined by scanning electron microscopy. Scanning electron microscopy showed that control cells had a smooth spherical surface, with a very few hemispherical projections of 1 um diameter. Thin-section transmission electron microscopy of the projections showed that they consisted of lipid droplets covered by a thin cytoplasmic matrix. On treatment with insulin, the number of these projections increased, and the optimal concentration of insulin caused a ten-fold increase in the number of cells with projections and the number of projections per cell. The number of projections decreased when the adipocytes were treated with phospholipase C or washed with buffer during lipogenesis with insulin. These observations may help in interpretation of biochemical data on the insulin receptor on the surface of adipocytes.
- 日本組織細胞化学会の論文
著者
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Kishino Yasuo
Department Of Nutrition School Of Medicine Tokushima University
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Kishino Yasuo
Department Of Nutrition School Of Medicine The University Of Tokushima
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Takahashi Yukari
Department Of Nutrition School Of Medicine Tokushima University
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Mizunuma Toshimi
Department Of Nutrition School Of Medicine Tokushima University
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TAKAHASHI Yukari
Department of Biological Mechanisms and Functions, Graduate School of Bioagricultural Sciences, Nagoya University
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KISHINO Yasuo
Department of Nutrition
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