Characterization of Folding Pathways of the Type-1 and Type-2 Periplasmic Binding Proteins MglB and ArgT

スポンサーリンク

概要

• 論文の詳細を見る

• The family of periplasmic binding proteins (PBPs) is believed to have arisen from a common ancestor and to have differentiated into two types. At first approximation, both types of PBPs have the same fold pattern, reflecting their common origin. How-ever, the connection between the main chains of a type 2 PBP is more complicated than a type 1 PBP's. We have been interested in the possibility that such structural changes affect the folding of PBPs. In this study, we have characterized the folding pathways of MglB (a type 1 PBP) and ArgT (a type 2 PBP) by using urea gradient gel electrophoresis, fast protein size-exclusion liquid chromatography and hydrophobic dye ANS binding assay. We found a distinct difference in folding between these two proteins. The folding of Mg1B followed a simple two-state transition model, whereas the folding of ArgT was more complicated.

• 社団法人 日本生化学会の論文

著者

• KASHIWAGI Kenji

  School of Pharmaceutical Sciences, University of Shizuoka

• FUKAMI-KOBAYASHI Kaoru

  National Institute of Genetics

• NISHIKAWA Ken

  National Institute of Genetics

• Shiba Kiyotaka

  Department Of Protein Engineering Cancer Institute Japanese Foundation For Cancer Research

• Noda Tetsuo

  Department Of Cell Biology Cancer Institute Japanese Foundation For Cancer Research

• Noguchi Hiroshi

  School Of Pharmaceutical Sciences And The Coe 21 Program University Of Shizuoka

関連論文

• Molecular Mechanism of Moderate Insulin Resistance in Adiponectin-Knockout Mice
• Different Effects of Oral Administration of Synthetic Trypsin Inhibitor on the Pancreas Between Cholecystokinin-A Receptor Gene Knockout Mice and Wild Type Mice
• Role of CCK-A Receptor for Pancreatic Function in Mice : A Study in CCK-A Receptor Knockout Mice
• Cloning and Functional Analysis of a Novel Aldo-Keto Reductase from Aloe arborescens(Biochemistry)
• P-129 Function of CHS-A and CHS-B in Ipomoea purpurea
• P-170 CRYSTAL STRUCTURE OF A NOVEL PLANT TYPE III POLYKETIDE SYNTHASE THAT PRODUCES PENTAKETIDE CHROMONE
• Characterization of Folding Pathways of the Type-1 and Type-2 Periplasmic Binding Proteins MgIB and ArgT
• Disruption of Cholecystokinin (CCK)-B Receptor Gene Did Not Modify Bile or Pancreatic Secretion of Pancreatic Growth : A Study in CCK-B Receptor Gene Knockout Mice
• The coiled tail of the round-headed spermatozoa appears during epididymal passage in GOPC-deficient mice
• Failure to assemble the peri-nuclear structures in GOPC deficient spermatids as found in round-headed spermatozoa
• Abnormal sperm morphology caused by defects in Sertoli cells of Cnot7 knockout mice
• Newly synthesized curcumin analog has improved potential to prevent colorectal carcinogenesis in vivo
• Adsorption Properties of a Gold-Binding Peptide Assessed by its Attachment to a Recombinant Apoferritin Molecule
• Differentiation Inducing Activities of Isocoumarins from Hydrangea Dulcis Folium
• Pancreatic β Cell Mass Preserved in Heterozygous PDK1 Knockout Mice
• Guide Oligonucleotide-Dependent DNA Linkage That Facilitates Controllable Polymerization of Microgene Blocks
• Nine New Triterpene Saponins, Polygalasaponins XXXIII-XLI from the Roots of Polygala fallax HEMSL.
• Prolyl Endopeptidase Inhibitors from the Roots of Lindera strychnifolia F. VILL
• P-95 ENGINEERED BIOSYNTHSIS OF "SUPRANATURAL STEROIDS"
• Apoptosis-Inducing Activity of Lipid Derivatives of Gallic Acid
• Structure Function Analysis of Novel Type III Polyketide Synthases from Arabidopsis thaliana(Biochemistry)
• Synthesis of Artificial Proteins That Have a Cell-Attaching Motif and Titanium-Binding Motif
• Construction and Characterization of Chimeric Proteins Composed of Type-1 and Type-2 Periplasmic Binding Proteins MglB and ArgT
• Biological Functionalization of Titanium Surfaces by Fusion Proteins between BMP-2 and Artificial Proteins Composed of Titanium-Binding Peptide Motifs
• Motif-programmed artificial protein induces apoptosis in several cancer cells by disrupting mitochondria
• Enzymatic Reactions by Five Chalcone Synthase Homologs from Hop (Humulus lupulus L.)
• P-10 ENGINEERED BIOSYNTHESIS OF PLANT POLYKETIDES
• Chalcone synthase superfamily of type III polyketide synthases from rhubarb (Rheum palmatum)
• The Functional Expression of the CHS-D and CHS-E Genes of the Common Morning Glory (Ipomoea purpurea) in Escherichia coli and Characterization of Their Gene Products
• SYNTHESIS OF FUNCTIONAL GENES BY MIXING MICROGENES(From Molecules to Life-Like Systems & RNA World, International Symposium on Origins of Life and Astrobiology)
• Prospective randomized phase II study determines the clinical usefulness of genetic biomarkers for sensitivity to primary chemotherapy with paclitaxel in breast cancer
• Acyl-CoA : Cholesterol Acyltransferase(ACAT) Inhibitors from Ilex spp.
• 1E1348 ヒトプロテオームに存在する新規ホールド数の見積もり(ゲノム生物学、生命情報科学,第49回日本生物物理学会年会)
• Integrative Annotation of 21,037 Human Genes Validated by Full-Length cDNA Clones
• The H-Invitational Database (H-InvDB), a comprehensive annotation resource for human genes and transcripts
• Characterization of Folding Pathways of the Type-1 and Type-2 Periplasmic Binding Proteins MglB and ArgT

もっと見る 閉じる

スポンサーリンク