A New Method for Rapid Detection of the Mutant Allele for Chediak-Higashi Syndrome in Japanese Black Cattle
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概要
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Chediak-Higashi syndrome (CHS) is an autosomal recessive hereditary disorder in Japanese Black cattle, caused by a mutation of the Lyst gene. So far, the mutation has been detected by PCR-restriction fragment length polymorphism (PCR-RFLP) analysis. However, this method is disadvantaged by its low-throughput performance. Here, we report an alternative method involving real-time PCR with TaqMan minor groove binder probes, which shortens the total assay time by more than 120 min, analyzing 10 samples in a duplicated manner. Using this method, we examined 102 Japanese Black cattle and found that 8.8% of the cattle were CHS-carriers. These data indicate that our technique is useful for routine diagnostic testing for CHS in Japanese Black cattle.
著者
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Ikeda Masahiro
Department Of Cardiovascular Surgery Fukui Cardiovascular Center
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Sonoda Hiroko
Department Of Veterinary Pharmacology Faculty Of Agriculture University Of Miyazaki
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Kitahara Go
Laboratory Of Theriogenology Faculty Of Agriculture University Of Miyazaki
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KOBAYASHI Ikuo
Sumiyoshi Livestock Science Station, Field Science Center, Faculty of Agriculture, University of Miyazaki, Miyazaki 889–2192, Japan
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ABDEEN Ahmed
Department of Veterinary Pharmacology, Faculty of Agriculture, University of Miyazaki, Miyazaki 889–2192, Japan
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