Protective Effects of Apomorphine against Zinc-Induced Neurotoxicity in Cultured Cortical Neurons
スポンサーリンク
概要
- 論文の詳細を見る
There is evidence that excessive zinc (Zn2+) release from presynaptic terminals following brain injuries such as ischemia and severe epileptic seizures induces neuronal cell death. Apomorphine (Apo), a dopamine receptor agonist, has been shown to have pleiotropic biological functions. In this study, we investigated whether Apo protects cultured cortical neurons from neurotoxicity provoked by excessive Zn2+ exposure. Pretreatment with Apo dose- and time-dependently ameliorated Zn2+ neurotoxicity. In addition, pretreatment with Apo prevented intracellular nicotinamide adenine dinucleotide (NAD+) and ATP depletion caused by Zn2+ exposure. Dopamine receptor antagonists did not influence Apo protection against Zn2+ neurotoxicity. Apo is shown to be autoxidized to produce oxidized products such as reactive oxygen species and quinones. N-Acetylcysteine, a thiol compound, partially reduced Apo protection. Entry of Zn2+ into neurons is thought to be a critical step of Zn2+ neurotoxicity. Interestingly, we found that pretreatment with Apo decreased elevation of intracellular Zn2+ levels after Zn2+ exposure and induced mRNA expression of the zinc transporter ZnT1, which transports intracellular Zn2+ out of cells, and metallothionein. Taken together, these results suggest that the protective effects of Apo are regulated, at least in part, by its oxidized products, and preventing intracellular accumulation of Zn2+ contributes to Apo protection against Zn2+ neurotoxicity.
著者
-
Adachi Tetsuo
Laboratory Of Clinical Pharmaceutics Gifu Pharmaceutical University
-
HARA Hirokazu
Laboratory of Clinical Pharmaceutics, Gifu Pharmaceutical University
-
Kamiya Tetsuro
Laboratory of Clinical Pharmaceutics, Gifu Pharmaceutical University
-
Maeda Asuka
Laboratory of Clinical Pharmaceutics, Gifu Pharmaceutical University
関連論文
- ELISA for human tissue-unspecific (liver/bone/kidney) alkaline phosphatase.
- Variable glyceryl dinitrate formation as a function of glutathione S-transferase.
- Variable Glyceryl Dinitrate Formation as a Function of Glutathione S-Transferase
- Protective Role of Extracellular Superoxide Dismutase in Hemodialysis Patients.
- Polymorphism of Extracellular Superoxide Dismutase(EC-SOD) Gene : Relation to the Mutation Responsible for High EC-SOD Level in Serum.
- POLYMORPHISM OF EXTRACELLULAR SUPEROXIDE DISMUTASE (EC-SOD) GENE : RELATION TO THE MUTATION RESPONSIBLE FOR HIGH EC-SOD LEVEL IN SERUM
- Circulating extracellular superoxide dismutase antagonizes blood pressure elevation in patients with coronary artery disease
- Angiotensin Receptor Blocker Improves Coronary Flow Velocity Reserve in Hypertensive Patients : Comparison with Calcium Channel Blocker
- PJ-425 Serum Soluble Lectin-Like Oxidized Low-Density Lipoprotein Receptor-1 Correlated with Cxidative Stress Markers in Stable Coronary Artery Disease(Atherosclerosis, clinical-12, The 71st Annual Scientific Meeting of the Japanese Circulation Society)
- PE-015 Decreased Plasma Extracellular Superoxide Dismutase Level in Patients with Vasospastic Angina(Angina pectoris, basic/clinical-3 (IHD) PE3,Poster Session (English),The 70th Anniversary Annual Scientific Meeting of the Japanese Circulation Society)
- OJ-177 Gene Transfer of Human Extracellular Superoxide Dismutase Prevents Monocrotaline-Induced Pulmonary Hypertension in Rats(Pulmonary circulation-2 (H) OJ30,Oral Presentation (Japanese),The 70th Anniversary Annual Scientific Meeting of the Japanese Cir
- Upregulation of Vascular Extracellular Superoxide Dismutase in Patients With Congestive Heart Failure (Heart Failure, Clinical 16 (M), The 69th Annual Scientific Meeting of the Japanese Circulation Society)
- Cuff-induced Neointimal Formation is Reduced by Extracellular Superoxide Dismutase(Atherosclerosis, Basic 6 (IHD), The 69th Annual Scientific Meeting of the Japanese Circulation Society)
- Hyperhomocysteinemia is Associated With Human Coronary Atherosclerosis Through the Reduction of the Ratio of Endothelium-Bound to Basal Extracellular Superoxide Dismutase
- Overexpression of EC-SOD suppresses endothelial-cell-mediated LDL oxidation.
- Analysis of inquiries about prescriptions at the Pharmacy of Gifu Pharmaceutical University.
- Effects of Homocysteine on the Binding of Extracellular-Superoxide Dismutase to the Endothelial Cell Surface.
- Plasma Extracellular-Superoxide Dismutase in Healthy Newborns and Infants/Children.
- Heparin-Induced Release of Extracellular-Superoxide Dismutase Form (V) to Plasma
- High blood superoxide dismutase (SOD) states in patients with diabetes mellitus. : Dependence on extracellular (EC)-SOD.
- Correlation between Oxidized LDL and Granulocyte Elastase in Patients with Diabetes Mellitus.
- Increase of Urinary Extracellular-Superoxide Dismutase Level Correlated with Cyclic Adenosine Monophosphate.
- Heparin-stimulated expression of extracellular-superoxide dismutase in human fibroblasts.
- Changes in the Heparin Affinity of Extracellular-Superoxide Dismutase in Patients with Coronary Artery Atherosclerosis.
- Changes in the Heparin Affinity of Extracellular-Superoxide Dismutase in Patients with Coronary Artery Atherosclerosis
- Substitution of glycine for arginine-213 in extracellular-superoxide dismutase impairs affinity for heparin and endothelial cell surface.
- An arginine-213 to glycine mutation in human extracellularsuperoxide dismutase reduces susceptibility to trypsin-like proteinases.
- An Arginine-213 to Glycine Mutation in Human Extracellular-Superoxide Dismutase Reduces Susceptibility to Trypsin-Like Proteinases
- Molecular analysis of extracellular-superoxide dismutase gene associated with high level in serum.
- MOLECULAR ANALYSIS OF EXTRACELLULAR-SUPEROXIDE DISMUTASE GENE ASSOCIATED WITH HIGH LEVEL IN SERUM
- Heparin-Affinity of Human Extracellular-Superoxide Dismutase in the Brain
- The alkaline phosphatase in human plexus chorioideus.
- Internalization of human extracellular-superoxide dismutase by bovine aortic endothelial cells.
- The nature of heterogeneous components of extracellular-superoxide dismutase purified from human umbilical cords.
- Quantitative Analysis of Extracellular-Superoxide Dismutase in Serum and Urine by ELISA with Monoclonal Antibody.
- Extracellular superoxide dismutase level in chronic hemodialysis patients.
- Heparin-induced release of extracellular-superoxide dismutase form(V) to plasma.
- Quantitative and qualitative changes of extracellular-superoxide dismutase in patients with various diseases.
- Pedigree of serum extracellular-superoxide dismutase level.
- Effect of Hypoxia Mimetic Cobalt Chloride on the Expression of Extracellular-Superoxide Dismutase in Retinal Pericytes
- Chemical Nature of Intestinal-Type Alkaline Phosphatase in Human Kidney.
- Pathologic Background of Abnormal Serum Amyloid A and Interleukin-6 Levels Revealed by a Piecewise Linear Regression Model in the Population of Diabetic Patients
- Propolis Suppresses CdCl2-Induced Cytotoxicity of COS7 Cells through the Prevention of Intracellular Reactive Oxygen Species Accumulation
- Extracellular-Superoxide Dismutase Expression in COS7 Cells Exposed to Cadmium Chloride
- Protective Effects of Apomorphine against Zinc-Induced Neurotoxicity in Cultured Cortical Neurons