On the Isolation of Mitochondria with High Respiratory Control from Guinea Pig Pancreas

概要

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In spite of the great attention paid to the study of the peculiarities of pancreas metabolism, there is no report in the literature concerning oxidative phosphorylation of pancreas mitochondria, owing mainly to the difficulty encountered in preparation of pancreas mitochondria, which are physiologically intact. The present results show an isolation method of mitochondria with a high respiratory control from guinea pig pancreas which is comparable with those prepared from other tissues. Care was mainly taken to separate the brown pellets as quickly as possible, which were precipitated from pancreas homogenates between 2,000×g for 7 minutes and 6,000×g for 10 minutes. The mitochondrial fractions prepared by this method consisted primarily of mitochondria with high phosphorylation efficiencies and were much less contaminated by nonmitochondrial particles, especially zymogen, than those obtained by other procedures. The quality of the mitochondrial preparation is considered acceptable as a starting material for biochemical work.
Japan Society of Clinical Chemistryの論文