Simultaneous Determination of Aluminum and Iron in Serum by Spectrophotometric Detection-Ion-Pair Reversed-Phase High-Performance Liquid Chromatography with 2, 2'-Dihydroxyazobenzene

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A highly sensitive and selective method using high-performance liquid chromatography for simultaneous mesurement of aluminum and iron in human serum of patients on chronic hemodialysis is described. Serum aluminum and iron are liberated by acid hydrolysis followed by deproteinization, and the chelates of the metal ions with 2, 2'-dihydroxyazobenzene are formed. A 100μl of the solution is injected into the HPLC column. The metal chelates are separated on an ODS column in an ion-pair reversed-phase mode and detected by the spectrophotometer. The proposed method enables sufficient recovery and reproducibility, and consists of a simple, sensitive and selective method for serum aluminum and iron measurements. A good agreement exists between this method and conventional method, based on graphite furnace atomic absorption spectrophotometry for aluminum and spectrophotometry for iron, respectively. It was confirmed that patients on chronic hemodialysis are prone to hyperaluminemia and hypoferrism. Further more, a negative correlation was observed between the contents of aluminum and iron in their sera.
Japan Society of Clinical Chemistryの論文