A new colorimetric method for the determination of plasma Lecithin Cholesterol Acyltransferase activity

概要

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A new method was developed to improve the linearity of plasma LCAT reaction by adding synthetic dipalmitoyl lecithin sol in incubation medium and subsequently measuring the change of free cholesterol contents after incubation by combined enzymatic method using cholesterol oxidase and peroxidase.<BR>The optimum concentration of dipalmitoyl lecithin in sol which was added into the fresh plasma was found in the range of 1.0-3.0mg/ml.<BR>The time course of the plasma LCAT reaction in this system proceeded almost linearly until two hours' incubation when plasma containing less than 120 n moles/ml/hr of their LCAT activities were used.<BR>The following additional data were obtained R=78 n moles/ml/hr, S. D.=4.73 n moles/ ml/hr and C. V.=6.0%. The correlation coefficient of the present method to GLC method was 0.83.<BR>The present method was found to be simpler than other techniques such as GLC or isotope methods and therefore may be suitable for the routine assays of plasma LCAT activities in the clinical laboratories.
Japan Society of Clinical Chemistryの論文