Rapid and Microdetermination of Carbon Monoxide in Blood using Oxygen Electrode and Erythrocytes

概要

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A new method for determination of carbon monoxide (CO) in blood by using polarographic oxygen (O2) electrode and erythrocytes was deviced. This method consists of following procedures; (1) 15 μl of CO-blood was added into 1 ml of O2-depleted water (O2 4 μg/ml) in the closed glass cell fixed with O2 electrode. Deoxyhemoglobin (HHb) in CO-blood was saturated with O2 dissolved in the O2-depleted water and converted to oxyhemoglobin (HbO2). (2) By addition of 10 μl of 10% K3Fe(CN)6 solution, HbO2 and carboxyhemoglobin (HbCO) in the reaction mixture were changed to methemoglobin, and O2 from HbO2 and CO from HbCO were released into reaction mixture. (3) Then, 30 μl of 30% NaCI solution was added to become isotonicity, and 10 μl of suspension of erythrocytes was added. The released CO from HbCO was entered into erythrocytes, and exchanged with O2 of HbO2 in erythrocytes. Equimolar O2 to CO was released to the reaction mixture. From the increase of O2 in the reaction mixture, the amount of CO in CO-blood was calculated. This method requirs only 15 μl of CO-blood and 4 min for each determination.
Japan Society of Clinical Chemistryの論文