Adsorption of Lysozyme on Biomass Charcoal Powder Prepared from Plant Biomass Wastes

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概要

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• The adsorption of chicken egg-white lysozyme onto biomass charcoal powder (BCP), which was prepared from plant biomass wastes such as dumped adzuki beans and bamboos by pyrolysis without combustion under nitrogen atmosphere and grinding with a jet mill, has been examined. The adsorption isotherms were successfully correlated by the Freundlich equation. The amount of lysozyme adsorbed on BCP was dramatically dependent upon the solution pH and temperature. The maximum adsorption was observed near neutral pH, suggesting that the adsorption was mainly attributable to the electrostatic interaction between the positive charge of enzymes and the negative charge on the surface of BCP. Plots of the amount of lysozyme adsorbed versus temperature exhibited an optimum temperature. The BCP was characterized by Raman spectroscopy, SEM, specific surface area, ζ potential, solid-state CP/MAS 13C-NMR spectroscopy, and X-ray photoelectron spectroscopy.

著者

• NORITOMI Hidetaka

  Department of Applied Chemistry, Tokyo Metropolitan University

• IWAI Daiki

  Department of Applied Chemistry, Tokyo Metropolitan University

• KAI Ryotaro

  Department of Applied Chemistry, Tokyo Metropolitan University

• TANAKA Masahiko

  EEN Co., Ltd.

• KATO Satoru

  EEN Co., Ltd.

• Kato Satoru

  Department of agriculture, Kinki University

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