Electrochemical Characterization of Enzyme and Immunoglobulin G Patterned Using Microcontact Printing
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概要
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Microcontact printing (μCP) with various proteins has been widely applied to biosensors and cell biology research. However, the mechanism of protein patterning in the μCP process is not clear in detail. We have electrochemically estimated enzyme concentration on glass slide patterned with μCP technique. We also estimated the enzyme concentration at the surface of poly(dimethylsiloxane) (PDMS), which corresponded to the enzyme activity at the PDMS stamp just before μCP. Our result suggested that it was possible to transfer enzyme monolayer from PDMS to glass surfaces with 100% efficiency with μCP. Immunoglobulin G (IgG) patterned with μCP was also characterized by tagging with enzyme labeled anti-IgG. Scanning electrochemical microscopy (SECM) image was obtained to visualize line and space pattern of IgG monolayer.
著者
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Yamada Hiroshi
Department of Analytical Chemistry, Faculty of Pharmaceutical Sciences, Toho University
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Yasukawa Tomoyuki
Graduate School Of Engineering Tohoku University
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Luo Hong
School Of Chemistry And Chemical Engineering Southwest China Normal University
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SHIKU Hitoshi
Graduate School of Environmental Studies, Tohoku University
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Shiku Hitoshi
Graduate School Of Environmental Studies Tohoku University
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Matsue Tomokazu
Graduate School Of Environmental Studies Tohoku University
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KUMAGAI Ayako
Graduate School of Environmental Studies, Tohoku University
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TAKAHASHI Yasufumi
Graduate School of Environmental Studies, Tohoku University
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