ヘモグロビン吸着カーボンフェルトによる酸素の生体触媒的電解還元と、アジ化物による阻害
スポンサーリンク
概要
- 論文の詳細を見る
Hemoglobin (Hb) was adsorbed onto a carbon-felt (CF), which is a microelectrode ensemble of microcarbon fiber (ca. 7 µm diameter) and possesses a random three-dimensional structure. Direct electron transfer between Hb heme and the CF was achieved without any electron mediating species and special materials. The Hb-adsorbed CF (Hb-CF) showed a pair of well-defined cyclic voltammetric peaks with the formal potential of −0.221 V (vs. Ag/AgCl) at pH 5.0 (0.1 M phosphate/citrate buffer), which is attributed to Hb Fe(III)/Fe(II) redox couple. The apparent heterogeneous electron-transfer rate constant (ks) was estimated to be 14.6 s−1. Furthermore, the Hb-CF exhibited an excellent bioelectrocatalytic activity for the reduction of O2. This bioelectrocatalytic activity was inhibited by azide, which binds to active heme center of Hb.
著者
-
Hasebe Yasushi
Department Of Applied Chemistry Faculty Of Engineering Saitama Institute Of Technology
-
WANG Yue
School of Chemical Engineering, University of Science and Technology LiaoNing
-
HASEBE Yasushi
Department of Life Science and Green Chemistry, Saitama Institute of Technology
関連論文
- EFFECTS OF VALPROIC ACID ON SPINAL REFLEXES AND [^3H]MUSCIMOL AND [^3H]DIAZEPAM BINDING IN BRAIN MEMBRANES IN RATS
- Structure-Activity Relationships of Phenylethylamine Analogs in Their Serotonergic Depressant Effects on the Spinal Monosynaptic Reflex in Rats
- Enhancement of Spinal Monosynaptic Reflexes with Phenylethylamine and Related Drugs through Descending Noradrenergic Neurons
- Chemically Amplified Flow-Injection Analysis of Polyphenolic Compounds using Immobilized-Tyrosinase Reactor and L-Ascorbic Acid, Based on Substrate Recycling
- Formation of aniline-bonded catechols in aqueous solution, and their electrochemical behaviors at carbon felt electrode
- Carbon Felt-based Bioelectrocatalytic Flow Detectors : Highly Sensitive Amperometric Determination of Hydrogen Peroxide Using Adsorbed Peroxidase and Thionine
- Carbon felt-based reagentless amperometric hydrogen peroxide biosensor usingco-immobilized toluidine blue O and horseradish peroxidase
- Carbon-felt-based Bioelectrocatalytic Flow-detectors : Role of Ultrasound Irradiation during the Adsorption of Horseradish Peroxidase and Thionine for a Highly Sensitive Amperometric Determination of H_2O_2
- Carbon-felt-based Bioelectrocatalytic Flow-detectors : Optimization of the Adsorption Conditions of Horseradish Peroxidase and Thionine onto Carbon-felt for Highly Sensitive Amperometric Determination of H_2O_2
- Chemically amplified current responese of FAD-enzyme electrodes based on the intermediate regeneration induced by L-ascorbate
- アスコルビン酸による酵素中間体再生反応に基づくFAD-酵素固定化電極の化学増幅電流応答〔英文〕 (バイオエレクトロケミストリ-の新展開)
- _L-Ascorbate sensor with polypyrrole coated carbon felt membrane, electropolymerized in a cucumber juice solution
- Functional Conversion of Metallo-Proteins : Novel Ascorbate Oxidase Activity of Copper-Monoamino Oxidase Induced by Exogenous Ligands
- ヘモグロビン吸着カーボンフェルトによる酸素の生体触媒的電解還元と、アジ化物による阻害
- Glutamate Biosensor Using a DNA-Cu(II)/polyamine Membrane as a Novel Electrocataytic Layer for Cathodic Determination of Hydrogen Peroxide