多分化能を持つヒト断裂腱板組織由来細胞の解析

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Background: To prevent the retear of the rotator cuff, it is important to regenerate the tendon-to-bone interface after rotator cuff repair. Therefore, we targeted the cells from the torn human rotator cuff and investigated whether they have the potency for multilineage mesenchymal differentiation in vitro. Methods: The edges of the rotator cuff were harvested from patients who underwent arthroscopic rotator cuff repair, minced into pieces and cultured in a monolayer. We performed flow cytometric analyses to analyze cell surface markers. To induce multilineage differentiation, the cells were cultured in the osteogenic, adipogenic and chondrogenic differentiation media for 3 weeks. Then they were analyzed by histology, immunohistochemistry, and RT-PCR analyses. Results: In flow cytometric analyses the cells were positive for MSC-related markers; CD29, CD44, CD105 and CD166. On the other hand, the cells were negative for hematopoietic-lineage markers; CD14, CD45 and CD133. After 3 weeks of differentiation culture the cells showed an osteogenic, adipogenic and chondrogenic potential as evidenced by the immunohistochemistry and RT-PCR analyses. Discussion: This study showed that cells derived from the torn human rotator cuff have the capacity for multilineage mesenchymal differentiation. These results could have the way for tendon neoformation and regeneration using progenitor cells. The ability to generate bone-ligament interface is specifically of great importance, as it can be useful in healing torn tendons that require reattachment to bone. Further investigations for the mechanism of tenogenesis are necessary to find out. Conclusion: The human rotator cuff derived cells have the capacity for multilineage differentiation and contribute to the repair of rotator cuff.

多分化能を持つヒト断裂腱板組織由来細胞の解析

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