ヒト腱板組織由来細胞の軟骨分化の可能性

概要

論文の詳細を見る
Cartilage plays an important role in the restructure of tendon to bone interfaces after rotator cuff repair. In this study, we targeted the cells derived from human rotator cuff and investigated whether they have the potential for chondrogenic differentiation. The edges of the rotator cuff were harvested from patients who had sustained a rotator cuff tear and underwent arthroscopic rotator cuff repair. The harvested rotator cuff was minced, and the cells were cultured in monolayer culture. Flow cytometric analyses were performed using monoclonal antibodies. To induce chondrogenesis, a pellet culture was performed for three-dimensional culture for 3 weeks. About 2.5x10<SUP>5</SUP> cells were spun in serum free ITS-medium containing dexamethasone, ascorbate, proline, recombinant BMP-6 and TGF-β3. Chondrogenic differentiation was determined by gene expression using RT-PCR technique, histological and immunohistochemical analyses. Flow cytometric analyses showed positive immunoreactivities for CD29, 44, 105, 166. The other tested markers were negative. After 3 weeks the cells showed a chondrogenic differentiation as evidenced by expression of type II and X collagen in the RT-PCR analyses and the immunohistochemistry. These results showed that the cells derived from human rotator cuff have the potential for chondrogenic differentiation like the bone marrow stem cells. It is suggested that human rotator cuff derived cells have the possibility of participation in the restructure of tendon to bone interfaces.

ヒト腱板組織由来細胞の軟骨分化の可能性

スポンサーリンク

概要

著者

関連論文

スポンサーリンク