Evaluation of fluorescence polarization immunoassay for determination of cyclosporin in whole blood and its clinical relevance.
スポンサーリンク
概要
- 論文の詳細を見る
The fluorescence polarization immunoassay (FPIA) method for determination of cyclosporin in whole blood was evaluated and compared with the high-performance liquid chromatography (HPLC) and the radioimmunoassay (polyclonal-RIA) methods. The coefficients of variation for the within-run and between-run precisions were 5% and 7%, respectively, for samples ranging in concentration from 350 to 1, 750 ng/ml. Recoveries were determined by adding cyclosporin at concentrations from 250 to 1, 750ng/ml to patient whole blood ; they were, on the average, 98.1%. The calibration curve was stable throughout a 10-week study period. There was no clinically significant interference for the FPIA assay due to icterus, lipemia, or other commonly used drugs. There was considerable variation of the ratio of the FPIA result to the HPLC result, whereas there was a good correlation between the FPIA and the polyclonal-RIA results (Y=0.9X-27.2, r=0.983, n=45, P<0.005), when evaluated using specimens from renal transplant patients receiving cyclosporin orally. In clinical practice, although higher blood levels of cyclosporin by the FPIA do not always correspond to more abnormal results in the clinical laboratory tests, an elevation of the trough level by the FPIA should be useful as an indicator of the risk of occurrence of tissue toxicity. It was concluded that the FPIA assay for cyclosporin in whole blood showed sufficient reproducibility and reliability to be used for either emergency or routine analysis in clinical practice, and serves as a practical alternative to the polyclonal-RIA.
- 一般社団法人 日本臨床薬理学会の論文
著者
-
Ono Takeshi
Hospital Pharmacy Shiga University Of Medical Science
-
Minouchi Tokuzo
Hospital Pharmacy Shiga University Of Medical Science
-
Shimakawa Harumi
Hospital Pharmacy Shiga University Of Medical Science
-
Shibata Nobuhito
Hospital Pharmacy Shiga University Of Medical Science
-
HAYASHI Yukio
Hospital Pharmacy, Shiga University of Medical Science
-
SHIBATA Hidehiko
Hospital Pharmacy, Shiga University of Medical Science
関連論文
- ENHANCING EFFECT OF ABSORPTION PROMOTERS ON PERCUTANEOUS ABSORPTION OF A MODEL DYE (6-CARBOXYFLUORESCEIN) AS A POORLY ABBSORBABLE DRUG. III.^ HISTOLOGICAL STUDY AFTER ADDITION OF VARIOUS ABSORPTION PROMOTERS IN RATS
- EFFECT OF ADMINISTRATION ROUTE ON THE SELECTIVE LYMPHATIC DELIVERY OF CYCLOSPORIN A BY LIPID-SURFACTANT MIXED MICELLES
- ENHANCING EFFECT OF ABSORPTION PROMOTERS ON PERCUTANEOUS ABSORPTION OF A MODEL DYE (6-CARBOXYFLUORESCEIN) AS POORLY ABSORBABLE DRUGS. II. STUDY ON THE ABSORPTION PROMOTING EFFECT OF AZONE
- PROMOTION OF THE SELECTIVE LYMPHATIC DELIVERY OF CYCLOSPORIN A BY LIPID-SURFACTANT MIXED MICELLES
- ENHANCING EFFECT OF ABSORPTION PROMOTERS ON PERCUTANEOUS ABSORPTION OF A MODEL DYE (6-CARBOXYFLUORESCEIN) AS POORLY ABSORBABLE DRUGS. I. COMPARISON OF PLASMA LEVELS AFTER ADDITION OF VARIOUS ABSORPTION PROMOTERS IN RAT
- Fluctuation in Therapeutic Control Associated with Interchange of Prednisolone Tablet Formulations : Assessment of Bioequivalence by Dissolution Test
- Pharmacokinetic Behavior of Micafungin in Rats with Carbon Tetrachloride-Induced Acute Hepatic Failure(Biopharmacy)
- Simultaneous determination of glucocorticoids in plasma or urine by high-performance liquid chromatography with precolumn fluorimetric derivatization by 9-anthroyl nitrile
- A Predictive Model for Area Under the Concentration versus Time Curve of Cyclosporin A Using Several Routine Monitoring Results in Renal Transplant Patients
- Inhibition of Testosterone Biosynthesis in Testicular Microsomes by Various Imidazole Drugs. Comparative Study with Ketoconazole