A New Type Micro-Glucose Sensor Based on Amperometric Detection

概要

論文の詳細を見る
A variety of biosensors have been developed for thedetermination of substance of clinical importance, since Clark-type oxygen electrode-based enzyme sensors were demonstrated. For the practical applicationof biosensors, microfabrication of enzyme electrodes is very important because biosensors with the easy handling and disposable usage can be attained easily by miniaturization techniques. Here glucose oxidase has been taken as the enzyme to be immobilized, and the enzyme product, hydrogen peroxide, is oxidized by the electrode matrix. Therefore, the amperometric signal of the micro-enzyme electrode is related to the concentration of glucose. The 100% response of the micro-enzymese nsoris no longer than 3sec, and the determinable range for glucose is from 5x 10-7 to 10-2mol/1. The time stability of the sensors are satisfactory: The sensor response to 1mM glucose is within ±2%for several weeks. We have developed a unique electrochemical fabrication technique, simultaneous platinization and immobilization of enzyme molecules. In this technique enzyme is incorporated into electrolyte solution and is embodied on the growing surface of platinized platinum during electrolysis .
ライフサポート学会の論文