NMR study on inclusion complexes of L-phenylalanine and aspartame with cyclodextrins in aqueous solution.

スポンサーリンク

概要

• 論文の詳細を見る

• The interactions of Phenylalanine and Aspartame (<I>N</I>-L-α-Aspartyl-L-phenylalaninel-methyl ester, APM) with α- and β-cyclodextrins (CD) in aqueous solutions were investigated by <SUP>1</SUP>H and <SUP>15</SUP>N NMR techniques. The changes in chemical shift of CD protons on going from free CD to the mixture with phenylalanine and APM indicate that the phenyl ring is included in the cavity of CD in the complex states. The changes in the chemical shift of the β-protons of phenylalanine and APM following the binding to α- and β-CD indicate that the simple association constants (<I>K</I><SUB>a</SUB>) are not applicable to these systems except for the case of APM/β-CD (<I>K</I><SUB>a</SUB>=90), and that the large changes of the rotamer fractional population of APM side-chain occur for APM/β-CD system. The analysis with Asp–Phe(<SUP>15</SUP>N)–OMe suggests that the torsional angles of the main chain of APM are unchanged on the formation of the inclusion complex. The low-field shifts of amide proton and its nitrogen resonances of Asp–Phe(<SUP>15</SUP>N)–OMe in the complex formation suggest that the amide part of APM is hydrogen-bonded to the C2 or C3 hydroxyl group of β-CD.

著者

• Suzuki Ei-ichiro

  Central Research Laboratories Ajinomoto Co. Inc.

• Nagashima Nobuya

  Central Research Laboratories Ajinomoto Co.

• Takahashi Shin-ichiro

  Central Research Laboratories, Ajinomoto Co., Inc.

関連論文

• Dynamic Structures of Granulocyte Colony-Stimulating Factor Proteins Studied by Normal Mode Analysis : Two Domain-Type Motions in Low Frequency Modes
• Crystal Structure of N-(4-Methyl-5-oxo-1-imidazolin-2-yl)sarcosine Monohydrate
• Freezing Behavior of Intra- and Extracellular Water of Cultured Green Cells of Lavender as Measured by NMR(Biological Chemistry)
• Intermediate State during the Crystal Transition in Aspartame, Studied with Thermal Analysis, Solid-State NMR, and Molecular Dynamics Simulaiton
• Tertiary Structural Models of Human Interleukin-6 and Evaluation by Comparison with X-Ray and NMR Structures
• NMR study on inclusion complexes of L-phenylalanine and aspartame with cyclodextrins in aqueous solution.
• State of Water in Maillard-reacted Ovalbumin as Studied by Broad-line Pulsed NMR
• Crystallization of human recombinant interleukin-2.
• The number of water molecules bound to insulin.

スポンサーリンク