Synthesis of porcine motilin by fragment condensation using three protected peptide fragments.
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概要
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Synthesis of porcine motilin is described. Construction of the entire molecule of this hormone was carried out by the fragment condensation method in solution employing Rudinger's azide modification, and the resulting protected docosapeptide was deblocked by sodium–liquid ammonia procedure. Purification of the final product was performed by ion exchange chromatography on CM-Sephadex using ammonium acetate as eluent and gel filtration on BiO–Gel P-6. Purity of the synthetic docosapeptide was assessed by TLC in two different solvent systems and amino acid analysis. This polypeptide showed gastric motoractivity stimulating action on 30 min intravenous infusion in dogs in 5 ng/kg/min doses, which is characteristic to porcine motilin.
- 公益社団法人 日本化学会の論文
著者
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YANAIHARA NOBORU
Laboratory of Bio-Organic Chemistry, Shizuoka College of Pharmacy
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Imagawa Kenichi
The First Research Institute, Tokushima Factory, Otsuka Pharmaceutical Co., Ltd.
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Shimizu Fumio
Laboratory of Bio-organic Chemistry, Shizuoka College of Pharmacy
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Mihara Shigeru
The First Research Institute, Tokushima Factory, Otsuka Pharmaceutical Co., Ltd.
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