Effect of Streptozotocin Administration on Somatostatin Content of Pancreas and Hypothalamus in Rats
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概要
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A radioimmunoassay (RIA) method for somatostatin (SRIF) utilizing rabbit antiserum against synthetic SRIF coupled with human serum α-globulin is described. Synthetic N<SUP>α</SUP>-tyrosylated SRIF was labelled with <SUP>125</SUP>I using the lactoperoxidase method and purified on a Sephadex G-10 column. This assay system was highly specific for SR IF and did not cross-react with hypothalamic trophic hormones, pituitary trophic hormones or gastrointestinal hormones.<BR>The effect of streptozotocin induced diabetes on the SRIF content was examined in the pancreas, the pancreatic islets, as well as the hypothalamus of rats. SRIF content in both the pancreas and islets of the diabetic rats was shown by RIA to have significantly increased. However, content in the hypothalamus of the diabetic rats did not differ from that of the control. The physiological and pathophysiological significance of the SRIF changes remains to be determined.
- 社団法人 日本内分泌学会の論文
著者
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Yanaihara Noboru
Laboratory Of Bioorganic Chemistry School Of Pharmaceutical Sciences University Of Shizuoka
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MAKINO HIDEICHI
Second Department of Internal Medicine, Chiba University School of Medicine
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KANATSUKA AZUMA
Second Department of Internal Medicine, Chiba University School of Medicine
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MATSUSHIMA YASUHISA
Second Department of Internal Medicine, Chiba University School of Medicine
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YAMAMOTO MASAHIRO
Second Department of Internal Medicine, Chiba University School of Medicine
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KUMAGAI AKIRA
Second Department of Internal Medicine, Chiba University School of Medicine
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YANAIHARA NOBORU
Laboratory of Bio-Organic Chemistry, Shizuoka College of Pharmacy
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