Peptide syntheses with proteinases. Fragment condensation of ZLeuGlnGlyOH or ZGlnGlyOH with HLeuValNH2 using metalloproteinases.

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概要

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• The crude preparations of microbial metalloproteinase from <I>Bacillus subtilis var. amyloliquefaciens</I> (Prolisin) catalyzed the condensation of ZLeuGlnGlyOH or ZGlnGlyOH with HLeuValNH<SUB>2</SUB> in the presence of the serineproteinase inhibitor from potatoes to give the corresponding Z-penta or tetra-peptide amide in good yields in an aqueous buffer solution. Two other metalloproteinases, from <I>Bacillus thermoproteoliticus</I> (Thermolysin or Thermoase) and <I>Streptomyces caespitosus</I> (Tacynase N), also catalyzed this reaction; however, they gave poorer results than Prolisin. The yields of the coupling products were affected most by the concentration of the substrates, among the several factors investigated. Addition of a small amount of an organic solvent sometimes promoted the yield for this method.

• 公益社団法人 日本化学会の論文

著者

• OHMORI Muneki

  Sagami Chemical Research Center

• Isowa Yoshikazu

  Sagami Chemical Research Center

• Ichikawa Tetsuya

  Sagami Chemical Research Center

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